ArticlePloS one2013
Identification of microRNA-Like RNAs in the filamentous fungus Trichoderma reesei by solexa sequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 93 citations in OpenAlex.
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- Global Analysis of microRNA-like RNAs Reveals Differential Regulation of Pathogenicity and Development inJournal of fungi (Basel, Switzerland) · 2024Article
- Discovering the hidden function in fungal genomes.Nature communications · 2024Review
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- Argonaute and Dicer are essential for communication betweenMicrobiology spectrum · 2024Article
- Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets.BMC genomics · 2023Article
- milR20 negatively regulates the development of fruit bodies inFrontiers in microbiology · 2023Article
- Cross-kingdom small RNA communication between plants and fungal phytopathogens-recent updates and prospects for future agriculture.RNA biology · 2023Review
- Comprehensive re-analysis of hairpin small RNAs in fungi reveals loci with conserved links.eLife · 2022Article
- Small RNA Analyses of aViruses · 2022Article
- RNA-Seq, Bioinformatic Identification of Potential MicroRNA-like Small RNAs in the Edible MushroomInternational journal of molecular sciences · 2022Article
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Computational Analysis of HTS Data and Its Application in Plant Pathology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions.Microbiology spectrum · 2021Article
- Vm-milR37 contributes to pathogenicity by regulating glutathione peroxidase gene VmGP in Valsa mali.Molecular plant pathology · 2021Article
- A Novel DCL2-Dependent Micro-Like RNAFrontiers in microbiology · 2021Article
- Identification and physiological function of one microRNA (Mycoscience · 2021Article
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
microRNAs (miRNAs) are non-coding small RNAs (sRNAs) capable of negatively regulating gene expression. Recently, microRNA-like small RNAs (milRNAs) were discovered in several filamentous fungi but not yet in Trichoderma reesei, an industrial filamentous fungus that can secrete abundant hydrolases. To explore the presence of milRNA in T. reesei and evaluate their expression under induction of cellulose, two T. reesei sRNA libraries of cellulose induction (IN) and non-induction (CON) were generated and sequenced using Solexa sequencing technology. A total of 726 and 631 sRNAs were obtained from the IN and CON samples, respectively. Global expression analysis showed an extensively differential expression of sRNAs in T. reesei under the two conditions. Thirteen predicted milRNAs were identified in T. reesei based on the short hairpin structure analysis. The milRNA profiles obtained in deep sequencing were further validated by RT-qPCR assay. Computational analysis predicted a number of potential targets relating to many processes including regulation of enzyme expression. The presence and differential expression of T. reesei milRNAs imply that milRNA might play a role in T. reesei growth and cellulase induction. This work lays foundation for further functional study of fungal milRNAs and their industrial application.
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