ArticlePloS one2013
Improved production of 2,3-butanediol in Bacillus amyloliquefaciens by over-expression of glyceraldehyde-3-phosphate dehydrogenase and 2,3-butanediol dehydrogenase.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 70 citations in OpenAlex.
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- Review
- Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol.Microbial cell factories · 2022Article
- Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13.Journal of microbiology (Seoul, Korea) · 2022Article
- Bioengineering for the industrial production of 2,3-butanediol by the yeast, Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2022Review
- Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production.Applied microbiology and biotechnology · 2021Review
- Rational Proteomic Analysis of a New DomesticatedFrontiers in microbiology · 2021Article
- The current strategies and parameters for the enhanced microbial production of 2,3-butanediol.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Elicitors of Plant Immunity Triggered by Beneficial Bacteria.Frontiers in plant science · 2020Review
- Microbial production of 2,3-butanediol for industrial applications.Journal of industrial microbiology & biotechnology · 2019Review
- Production of optically pure 2,3-butanediol from Miscanthus floridulus hydrolysate using engineered Bacillus licheniformis strains.World journal of microbiology & biotechnology · 2018Article
- Production of (2R, 3R)-2,3-butanediol using engineeredBiotechnology for biofuels · 2018Article
- Artificial consortium that produces riboflavin regulates distribution of acetoin and 2,3-butanediol byEngineering in life sciences · 2017Article
- Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity.Journal of industrial microbiology & biotechnology · 2017Article
- Strategies for efficient and economical 2,3-butanediol production: new trends in this field.World journal of microbiology & biotechnology · 2016Review
- In situ expression of (R)-carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)-phenyl-1,2-ethanediol in Candida parapsilosis CCTCC M203011.Microbial cell factories · 2016Article
- Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity.Plant molecular biology · 2016Review
- Impact of a Bacterial Volatile 2,3-Butanediol on Bacillus subtilis Rhizosphere Robustness.Frontiers in microbiology · 2016Article
- Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production.Biotechnology for biofuels · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreviously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-BD yield remains prohibitively low for commercial production. METHODOLOGY/PRINCIPAL
findingsIn the 2,3-butanediol metabolic pathway, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of 3-phosphate glyceraldehyde to 1,3-bisphosphoglycerate, with concomitant reduction of NAD(+) to NADH. In the same pathway, 2,3-BD dehydrogenase (BDH) catalyzes the conversion of acetoin to 2,3-BD with concomitant oxidation of NADH to NAD(+). In this study, to improve 2,3-BD production, we first over-produced NAD(+)-dependent GAPDH and NADH-dependent BDH in B. amyloliquefaciens. Excess GAPDH reduced the fermentation time, increased the 2,3-BD yield by 12.7%, and decreased the acetoin titer by 44.3%. However, the process also enhanced lactate and succinate production. Excess BDH increased the 2,3-BD yield by 16.6% while decreasing acetoin, lactate and succinate production, but prolonged the fermentation time. When BDH and GAPDH were co-overproduced in B. amyloliquefaciens, the fermentation time was reduced. Furthermore, in the NADH-dependent pathways, the molar yield of 2,3-BD was increased by 22.7%, while those of acetoin, lactate and succinate were reduced by 80.8%, 33.3% and 39.5%, relative to the parent strain. In fed-batch fermentations, the 2,3-BD concentration was maximized at 132.9 g/l after 45 h, with a productivity of 2.95 g/l·h. CONCLUSIONS/SIGNIFICANCE: Co-overexpression of bdh and gapA genes proved an effective method for enhancing 2,3-BD production and inhibiting the accumulation of unwanted by-products (acetoin, lactate and succinate). To our knowledge, we have attained the highest 2,3-BD fermentation yield thus far reported for safe microorganisms.
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