ArticlePLoS genetics2013
Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Cell cycle responses to Topoisomerase II inhibition: Molecular mechanisms and clinical implications.The Journal of cell biology · 2023Review
- Negative supercoils regulate meiotic crossover patterns in budding yeast.Nucleic acids research · 2022Article
- Sister chromatids separate during anaphase in a three-stage program as directed by interaxis bridges.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Role of Aurora B and Haspin kinases in the metaphase Topoisomerase II checkpoint.Cell cycle (Georgetown, Tex.) · 2021Review
- Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases.The Journal of cell biology · 2020Article
- A genome-wide RNAi screen identifies the SMC5/6 complex as a non-redundant regulator of a Topo2a-dependent G2 arrest.Nucleic acids research · 2019Article
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- Non-Catalytic Roles of the Topoisomerase IIα C-Terminal Domain.International journal of molecular sciences · 2017Review
- Article
- Mechanisms behind Topoisomerase II SUMOylation in chromosome segregation.Cell cycle (Georgetown, Tex.) · 2016Article
- Roles of eukaryotic topoisomerases in transcription, replication and genomic stability.Nature reviews. Molecular cell biology · 2016Review
- SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis.The Journal of cell biology · 2016Article
- Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate.Molecular and cellular biology · 2015Article
- Novel kinetochore function of Topoisomerase IIα.Cell cycle (Georgetown, Tex.) · 2015Article
- SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin.Cell cycle (Georgetown, Tex.) · 2015Article
- Effect of piperlongumine on drug resistance reversal in human retinoblastoma HXO-RB44/VCR and SO-Rb50/CBP cell lines.International journal of clinical and experimental pathology · 2015Article
- Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.Nature communications · 2014Article
- Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment.Nucleic acids research · 2014Article
- The analysis of mutant alleles of different strength reveals multiple functions of topoisomerase 2 in regulation of Drosophila chromosome structure.PLoS genetics · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
By necessity, the ancient activity of type II topoisomerases co-evolved with the double-helical structure of DNA, at least in organisms with circular genomes. In humans, the strand passage reaction of DNA topoisomerase II (Topo II) is the target of several major classes of cancer drugs which both poison Topo II and activate cell cycle checkpoint controls. It is important to know the cellular effects of molecules that target Topo II, but the mechanisms of checkpoint activation that respond to Topo II dysfunction are not well understood. Here, we provide evidence that a checkpoint mechanism monitors the strand passage reaction of Topo II. In contrast, cells do not become checkpoint arrested in the presence of the aberrant DNA topologies, such as hyper-catenation, that arise in the absence of Topo II activity. An overall reduction in Topo II activity (i.e. slow strand passage cycles) does not activate the checkpoint, but specific defects in the T-segment transit step of the strand passage reaction do induce a cell cycle delay. Furthermore, the cell cycle delay depends on the divergent and catalytically inert C-terminal region of Topo II, indicating that transmission of a checkpoint signal may occur via the C-terminus. Other, well characterized, mitotic checkpoints detect DNA lesions or monitor unattached kinetochores; these defects arise via failures in a variety of cell processes. In contrast, we have described the first example of a distinct category of checkpoint mechanism that monitors the catalytic cycle of a single specific enzyme in order to determine when chromosome segregation can proceed faithfully.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.