ArticleJournal of bacteriology2013
The syp enhancer sequence plays a key role in transcriptional activation by the σ54-dependent response regulator SypG and in biofilm formation and host colonization by Vibrio fischeri.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
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- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- Two enhancer binding proteins activate σeLife · 2023Article
- Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes.Microbiology spectrum · 2022Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- Hybrid Histidine Kinase BinK Represses Vibrio fischeri Biofilm Signaling at Multiple Developmental Stages.Journal of bacteriology · 2021Article
- The Bacterial Enhancer Binding Protein VasH Promotes Expression of a Type VI Secretion System in Vibrio fischeri during Symbiosis.Journal of bacteriology · 2020Article
- Nitric oxide inhibits biofilm formation by Vibrio fischeri via the nitric oxide sensor HnoX.Molecular microbiology · 2019Article
- Discovery of Calcium as a Biofilm-Promoting Signal for Vibrio fischeri Reveals New Phenotypes and Underlying Regulatory Complexity.Journal of bacteriology · 2018Article
- Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae.BMC research notes · 2018Article
- The Histidine Kinase BinK Is a Negative Regulator of Biofilm Formation and Squid Colonization.Journal of bacteriology · 2016Article
- CysK Plays a Role in Biofilm Formation and Colonization by Vibrio fischeri.Applied and environmental microbiology · 2015Article
- Signaling between two interacting sensor kinases promotes biofilms and colonization by a bacterial symbiont.Molecular microbiology · 2015Article
- Identification of a novel matrix protein that promotes biofilm maturation in Vibrio fischeri.Journal of bacteriology · 2015Article
- Assessing the function of STAS domain protein SypA in Vibrio fischeri using a comparative analysis.Frontiers in microbiology · 2015Article
- The putative oligosaccharide translocase SypK connects biofilm formation with quorum signaling in Vibrio fischeri.MicrobiologyOpen · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Biofilm formation by Vibrio fischeri is a complex process that requires multiple regulators. One such regulator, the NtrC-like response regulator SypG, controls biofilm formation and host colonization by V. fischeri via its impact on transcription of the symbiosis polysaccharide (syp) locus. SypG is predicted to activate syp transcription by binding to the syp enhancer (SE), a conserved sequence located upstream of four syp promoters. In this study, we performed an in-depth analysis of the sequences necessary for SypG to promote syp transcription and biofilm formation. We found that the SE sequence is necessary for SypG-mediated syp transcription, identified individual bases necessary for efficient activation, and determined that SypG is able to bind to syp promoter regions. We also identified SE sequences outside the syp locus and established that SypG recognizes these sequences as well. Finally, deletion of the SE sequence upstream of sypA led to defects in both biofilm formation and host colonization that could be restored by reintroducing the SE sequence into its native location in the chromosome. This work thus fills in critical gaps in knowledge of the Syp regulatory circuit by demonstrating a role for the SE sequence in SypG-dependent control of biofilm formation and host colonization and by identifying new putative regulon members. It may also provide useful insights into other bacteria, such as Vibrio vulnificus and Vibrio parahaemolyticus, that have syp-like loci and conserved SE sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.