Evidence map›Paper›PMID 24097806›Full record

ArticleTransactions of the Royal Society of Tropical Medicine and Hygiene2013

Susceptibility of tick cell lines to infection with Alkhumra haemorrhagic fever virus.

Tariq A Madani, El-Tayeb M E Abuelzein, Lesley Bell-Sakyi, Esam I Azhar, Hussein M S Al-Bar, Huda Abu-Araki, Ahmed M Hassan, Badr E Masri, Thomas G Ksiazek

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transactions of the Royal Society of Tropical Medicine and Hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Virus Discovery Using Tick Cell Lines.Evolutionary bioinformatics online · 2016
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Tariq A MadaniDepartment of Medicine, Faculty of Medicine, King Abdulaziz University, P.O. Box 80215, Jeddah 21589, Saudi Arabia.
El-Tayeb M E Abuelzein
Lesley Bell-Sakyi
Esam I Azhar
Hussein M S Al-Bar
Huda Abu-Araki
Ahmed M Hassan
Badr E Masri
Thomas G Ksiazek
King Abdulaziz University · SARoslin Institute · GBThe University of Texas Medical Branch at Galveston · US

Funding

Regulatory Compliance Core - Galveston National Laboratory BSL4 OperationsUC7AI094660 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Dennis A Bente · 2011 to 2026
$208.8M
Health, Safety and Training ServicesUC7AI070083 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI LEMON, STANLEY M. · 2006 to 2010
$49.5M
NIAID NIH HHS UC7 AI070083NIAID NIH HHS UC7 AI094660
6 · The paper itself

Abstract

backgroundAlthough Alkhumra haemorrhagic fever virus (AHFV) has been isolated from ticks, epidemiological data suggest that it is transmitted from livestock to humans by direct contact with animals or by mosquito bites, but not by ticks. This study was carried out to assess the ability of the virus to replicate in tick cells in vitro.

methodsAHFV was inoculated into cell lines derived from the hard ticks Hyalomma anatolicum (HAE/CTVM9) and Rhipicephalus appendiculatus (RAE/CTVM1) and the soft tick Ornithodoros moubata (OME/CTVM24). Inoculated cells were directly examined every week for 4 weeks by real-time reverse transcription PCR and by IFAT using polyclonal antibodies.

resultsAHFV RNA was detected in all three inoculated tick cell lines throughout the 4-week observation period at levels up to almost twice that of the inoculum, but none of them exhibited a cytopathic effect. AHFV antigen could be detected in all three cell lines by IFAT. Titration of tick cell culture suspension in LLC-MK2 cells yielded AHFV titres of 10(6.6) 50% tissue culture infective dose (TCID50)/ml for OME/CTVM24 and 10(5.5) TCID50/ml for RAE/CTVM1 cells after 4 weeks of culturing; no viable virus was detected in HAE/CTVM9 cells.

conclusionThis is the first description of propagation of AHFV in tick cells.

Indexed as

AnimalsCell LineCells, CulturedDisease SusceptibilityFluorescent Antibody Technique, IndirectHemorrhagic Fever, CrimeanHemorrhagic Fever Virus, Crimean-CongoHumansReal-Time Polymerase Chain ReactionTicksVirus ReplicationAlkhumra haemorrhagic fever virusIFATIndirect fluorescent antibody testPropagationReal time RT-PCRTick cell lines

Identifiers

PMID24097806
PMCPMC4357811
OpenAlexW2077125792

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.