ArticleScientific reports2013
Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions.Nature communications · 2026Article
- Mechanical communication and function regulation of immune cells.Fundamental research · 2026Review
- High-throughput spontaneous Raman imaging of biological specimens with water background reduction.Biomedical optics express · 2026Article
- Target cell adhesion limits macrophage phagocytosis and promotes trogocytosis.The Journal of cell biology · 2025Article
- Mechano-crosstalk between living and artificial cells.Nature communications · 2025Article
- Macrophages form dendrite-like pseudopods to enhance bacterial ingestion.The EMBO journal · 2025Article
- Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cellular Uptake and Trafficking of Lipid Nanocarriers Using High-Resolution Electron Microscopy.AAPS PharmSciTech · 2025Article
- Target cell adhesion limits macrophage phagocytosis and promotes trogocytosis.bioRxiv : the preprint server for biology · 2025Article
- Force-bearing phagocytic adhesion rings mediate the phagocytosis of surface-bound particles.Nature communications · 2025Article
- The role of bacterial size, shape and surface in macrophage engulfment of uropathogenic E. coli cells.PLoS pathogens · 2024Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens.Microbiology and molecular biology reviews : MMBR · 2022Review
- Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.Molecular microbiology · 2022Article
- Filopodia rotate and coil by actively generating twist in their actin shaft.Nature communications · 2022Article
- Induction of Filopodia During Cytomegalovirus Entry Into Human Iris Stromal Cells.Frontiers in microbiology · 2022Article
- Size-Dependent Internalization Efficiency of Macrophages from Adsorbed Nanoparticle-Based Monolayers.Nanomaterials (Basel, Switzerland) · 2021Article
- Tailoring Materials for Modulation of Macrophage Fate.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- Optogenetic manipulation of cellular communication using engineered myosin motors.Nature cell biology · 2021Article
- Stretching the Function of Innate Immune Cells.Frontiers in immunology · 2021Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To clear pathogens from host tissues or biomaterial surfaces, phagocytes have to break the adhesive bacteria-substrate interactions. Here we analysed the mechanobiological process that enables macrophages to lift-off and phagocytose surface-bound Escherichia coli (E. coli). In this opsonin-independent process, macrophage filopodia hold on to the E. coli fimbriae long enough to induce a local protrusion of a lamellipodium. Specific contacts between the macrophage and E. coli are formed via the glycoprotein CD48 on filopodia and the adhesin FimH on type 1 fimbriae (hook). We show that bacterial detachment from surfaces occurrs after a lamellipodium has protruded underneath the bacterium (shovel), thereby breaking the multiple bacterium-surface interactions. After lift-off, the bacterium is engulfed by a phagocytic cup. Force activated catch bonds enable the long-term survival of the filopodium-fimbrium interactions while soluble mannose inhibitors and CD48 antibodies suppress the contact formation and thereby inhibit subsequent E. coli phagocytosis.
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