ArticleThe EMBO journal2013
Structural basis for the MukB-topoisomerase IV interaction and its functional implications in vivo.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.Communications biology · 2025Article
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- Temporospatial control of topoisomerases by essential cellular processes.Current opinion in microbiology · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Single-nucleotide resolution detection of Topo IV cleavage activity in theFrontiers in microbiology · 2023Article
- Intersubunit and intrasubunit interactions driving the MukBEF ATPase.The Journal of biological chemistry · 2022Article
- The MukB-topoisomerase IV interaction mutually suppresses their catalytic activities.Nucleic acids research · 2022Article
- Article
- Mechanisms for Chromosome Segregation in Bacteria.Frontiers in microbiology · 2021Review
- Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP.Nature communications · 2020Article
- Architecture of the Escherichia coli nucleoid.PLoS genetics · 2019Article
- A folded conformation of MukBEF and cohesin.Nature structural & molecular biology · 2019Article
- MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.eLife · 2018Article
- The MukB-topoisomerase IV interaction is required for proper chromosome compaction.The Journal of biological chemistry · 2017Article
- Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.PLoS genetics · 2016Article
- MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.Nature communications · 2016Article
- The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF.Cell reports · 2015Article
- Molecular basis for SMC rod formation and its dissolution upon DNA binding.Molecular cell · 2015Article
- Article
- MukBEF, a chromosomal organizer.Journal of molecular microbiology and biotechnology · 2014Review
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Authors and funding
4 authors.
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Abstract
Chromosome partitioning in Escherichia coli is assisted by two interacting proteins, topoisomerase (topo) IV and MukB. MukB stimulates the relaxation of negative supercoils by topo IV; to understand the mechanism of their action and to define this functional interplay, we determined the crystal structure of a minimal MukB-topo IV complex to 2.3 Å resolution. The structure shows that the so-called 'hinge' region of MukB forms a heterotetrameric assembly with a C-terminal DNA binding domain (CTD) on topo IV's ParC subunit. Biochemical studies show that the hinge stimulates topo IV by competing for a site on the CTD that normally represses activity on negatively supercoiled DNA, while complementation tests using mutants implicated in the interaction reveal that the cellular dependency on topo IV derives from a joint need for both strand passage and MukB binding. Interestingly, the configuration of the MukB·topo IV complex sterically disfavours intradimeric interactions, indicating that the proteins may form oligomeric arrays with one another, and suggesting a framework by which MukB and topo IV may collaborate during daughter chromosome disentanglement.
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