ArticleEukaryotic cell2013
Involvement of Botrytis cinerea small GTPases BcRAS1 and BcRAC in differentiation, virulence, and the cell cycle.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Ras2 Is Responsible for the Environmental Responses, Melanin Metabolism, and Virulence ofJournal of fungi (Basel, Switzerland) · 2023Article
- Botrytis cinerea Transcription Factor BcXyr1 Regulates (Hemi-)Cellulase Production and Fungal Virulence.mSystems · 2022Article
- Combined transcriptome and proteome analysis of Bcfrp1 involved in regulating the biosynthesis of abscisic acid and growth inFrontiers in microbiology · 2022Article
- DNA Methyltransferases Regulate Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- NADPH Oxidase Regulates the Growth and Pathogenicity ofFrontiers in plant science · 2021Article
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- A Single Nucleotide Mutation in Adenylate Cyclase Affects Vegetative Growth, Sclerotial Formation and Virulence ofInternational journal of molecular sciences · 2020Article
- Article
- Global Proteomic Analysis of Lysine Crotonylation in the Plant PathogenFrontiers in microbiology · 2020Article
- Reactive oxygen species: A generalist in regulating development and pathogenicity of phytopathogenic fungi.Computational and structural biotechnology journal · 2020Review
- A Similar Secretome Disturbance as a Hallmark of Non-pathogenicFrontiers in microbiology · 2019Article
- Article
- BcXYG1, a Secreted Xyloglucanase fromPlant physiology · 2017Article
- Genetic alteration of UDP-rhamnose metabolism in Botrytis cinerea leads to the accumulation of UDP-KDG that adversely affects development and pathogenicity.Molecular plant pathology · 2017Article
- Orchestration of Morphogenesis in Filamentous Fungi: Conserved Roles for Ras Signaling Networks.Fungal biology reviews · 2015Article
- VdNUC-2, the Key Regulator of Phosphate Responsive Signaling Pathway, Is Required for Verticillium dahliae Infection.PloS one · 2015Article
- Measurement of apoptosis by SCANMicrobial cell (Graz, Austria) · 2014Article
- Small-GTPase-associated signaling by the guanine nucleotide exchange factors CpDock180 and CpCdc24, the GTPase effector CpSte20, and the scaffold protein CpBem1 in Claviceps purpurea.Eukaryotic cell · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small GTPases of the Ras superfamily are highly conserved proteins that are involved in various cellular processes, in particular morphogenesis, differentiation, and polar growth. Here we report on the analysis of RAS1 and RAC homologues from the gray mold fungus Botrytis cinerea. We show that these small GTPases are individually necessary for polar growth, reproduction, and pathogenicity, required for cell cycle progression through mitosis (BcRAC), and may lie upstream of the stress-related mitogen-activated protein kinase (MAPK) signaling pathway. bcras1 and bcrac deletion strains had reduced growth rates, and their hyphae were hyperbranched and deformed. In addition, both strains were vegetatively sterile and nonpathogenic. A strain expressing a constitutively active (CA) allele of the BcRAC protein had partially similar but milder phenotypes. Similar to the deletion strains, the CA-BcRAC strain did not produce any conidia and had swollen hyphae. In contrast to the two deletion strains, however, the growth rate of the CA-BcRAC strain was normal, and it caused delayed but well-developed disease symptoms. Microscopic examination revealed an increased number of nuclei and disturbance of actin localization in the CA-BcRAC strain. Further work with cell cycle- and RAC-specific inhibitory compounds associated the BcRAC protein with progression of the cell cycle through mitosis, possibly via an effect on microtubules. Together, these results show that the multinucleate phenotype of the CA-BcRAC strain could result from at least two defects: disruption of polar growth through disturbed actin localization and uncontrolled nuclear division due to constitutive activity of BcRAC.
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