ArticleApplied and environmental microbiology2013
Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 96 citations in OpenAlex.
- Article
- Advancing transcriptomic profiling of airborne bacteria.Applied and environmental microbiology · 2025Review
- Sampling efficiency and nucleic acid stability during long-term sampling with different bioaerosol samplers.Environmental monitoring and assessment · 2024Article
- Quiescence ofMicroorganisms · 2023Article
- Article
- Passive Bioaerosol Samplers: A Complementary Tool for Bioaerosol Research. A Review.Journal of aerosol science · 2022Article
- A review of the emergence of antibiotic resistance in bioaerosols and its monitoring methods.Re/views in environmental science and bio/technology · 2022Review
- Development and initial testing of an active low-power, ferroelectric film-based bioaerosol sampler.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2022Article
- Recent advancements in the measurement of pathogenic airborne viruses.Journal of hazardous materials · 2021Review
- Paper-Based Airborne Bacteria Collection and DNA Extraction Kit.Biosensors · 2021Article
- Impact of sampling and storage stress on the recovery of airborne SARS-CoV-2 virus surrogate captured by filtration.Journal of occupational and environmental hygiene · 2021Article
- Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan.BMC microbiology · 2021Article
- Physical collection and viability of airborne bacteria collected under electrostatic field with different sampling media and protocols towards rapid detection.Scientific reports · 2021Article
- Multiple relationships between aerosol and COVID-19: A framework for global studies.Gondwana research : international geoscience journal · 2021Article
- Aerosol agitation: Quantifying the hydrodynamic stressors on particulates encapsulated in small droplets.Physical review fluids · 2021Article
- A Diffusion Model to Quantify Membrane Repair Process inFrontiers in microbiology · 2021Article
- Aerosolization and recovery of viable murine norovirus in an experimental setup.Scientific reports · 2020Article
- Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France.International journal of environmental research and public health · 2020Article
- On airborne transmission and control of SARS-Cov-2.The Science of the total environment · 2020Article
- Bioaerosol Sampling: Classical Approaches, Advances, and Perspectives.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report here that stress experienced by bacteria due to aerosolization and air sampling can result in severe membrane impairment, leading to the release of DNA as free molecules. Escherichia coli and Bacillus atrophaeus bacteria were aerosolized and then either collected directly into liquid or collected using other collection media and then transferred into liquid. The amount of DNA released was quantified as the cell membrane damage index (ID), i.e., the number of 16S rRNA gene copies in the supernatant liquid relative to the total number in the bioaerosol sample. During aerosolization by a Collison nebulizer, the ID of E. coli and B. atrophaeus in the nebulizer suspension gradually increased during 60 min of continuous aerosolization. We found that the ID of bacteria during aerosolization was statistically significantly affected by the material of the Collison jar (glass > polycarbonate; P < 0.001) and by the bacterial species (E. coli > B. atrophaeus; P < 0.001). When E. coli was collected for 5 min by filtration, impaction, and impingement, its ID values were within the following ranges: 0.051 to 0.085, 0.16 to 0.37, and 0.068 to 0.23, respectively; when it was collected by electrostatic precipitation, the ID values (0.011 to 0.034) were significantly lower (P < 0.05) than those with other sampling methods. Air samples collected inside an equine facility for 2 h by filtration and impingement exhibited ID values in the range of 0.30 to 0.54. The data indicate that the amount of cell damage during bioaerosol sampling and the resulting release of DNA can be substantial and that this should be taken into account when analyzing bioaerosol samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.