ArticleApplied and environmental microbiology2013
Diverse broad-host-range plasmids from freshwater carry few accessory genes.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- Plasmid-Host Networks Reveal Persistent Mobile Elements Across Wastewater Treatment.Nature communications · 2026Article
- Selective shifts in mobile antibiotic resistance genes under carbamazepine exposure in wastewater microbiomes.ISME communications · 2026Article
- Not So Mosaic After All? The Core Genomes of IncP-1 Plasmids Evolve Predominantly Through Vertical Transmission.bioRxiv : the preprint server for biology · 2025Article
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- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- Encounter rates and engagement times limit the transmission of conjugative plasmids.PLoS genetics · 2025Article
- Polluted wetlands contain multidrug-resistance plasmids encoding CTX-M-type extended-spectrum β-lactamases.Plasmid · 2023Article
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- Hitherto-Unnoticed Self-Transmissible Plasmids Widely Distributed among Different Environments in Japan.Applied and environmental microbiology · 2022Article
- Deciphering the Regulatory Circuits of RA3 Replication Module - Mechanisms of the Copy Number Control.International journal of molecular sciences · 2022Article
- Giant Starship Elements Mobilize Accessory Genes in Fungal Genomes.Molecular biology and evolution · 2022Article
- Characterization of a Conjugative Multidrug Resistance IncP-2 Megaplasmid, pPAG5, from a Clinical Pseudomonas aeruginosa Isolate.Microbiology spectrum · 2022Article
- Long-Range PCR Reveals the Genetic Cargo of IncP-1 Plasmids in the Complex Microbial Community of an On-Farm Biopurification System Treating Pesticide-Contaminated Wastewater.Applied and environmental microbiology · 2022Article
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- On-Farm Anaerobic Digestion of Dairy Manure Reduces the Abundance of Antibiotic Resistance-Associated Gene Targets and the Potential for Plasmid Transfer.Applied and environmental microbiology · 2021Article
- Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance.Applied and environmental microbiology · 2021Article
- Biosolids as a Source of Antibiotic Resistance Plasmids for Commensal and Pathogenic Bacteria.Frontiers in microbiology · 2021Article
- Modeling of shotgun sequencing of DNA plasmids using experimental and theoretical approaches.BMC bioinformatics · 2020Article
- Plant Species-Dependent Increased Abundance and Diversity of IncP-1 Plasmids in the Rhizosphere: New Insights Into Their Role and Ecology.Frontiers in microbiology · 2020Article
- PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera.Frontiers in microbiology · 2020Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
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Abstract
Broad-host-range self-transferable plasmids are known to facilitate bacterial adaptation by spreading genes between phylogenetically distinct hosts. These plasmids typically have a conserved backbone region and a variable accessory region that encodes host-beneficial traits. We do not know, however, how well plasmids that do not encode accessory functions can survive in nature. The goal of this study was to characterize the backbone and accessory gene content of plasmids that were captured from freshwater sources without selecting for a particular phenotype or cultivating their host. To do this, triparental matings were used such that the only required phenotype was the plasmid's ability to mobilize a nonconjugative plasmid. Based on complete genome sequences of 10 plasmids, only 5 carried identifiable accessory gene regions, and none carried antibiotic resistance genes. The plasmids belong to four known incompatibility groups (IncN, IncP-1, IncU, and IncW) and two potentially new groups. Eight of the plasmids were shown to have a broad host range, being able to transfer into alpha-, beta-, and gammaproteobacteria. Because of the absence of antibiotic resistance genes, we resampled one of the sites and compared the proportion of captured plasmids that conferred antibiotic resistance to their hosts with the proportion of such plasmids captured from the effluent of a local wastewater treatment plant. Few of the captured plasmids from either site encoded antibiotic resistance. A high diversity of plasmids that encode no or unknown accessory functions is thus readily found in freshwater habitats. The question remains how the plasmids persist in these microbial communities.
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