ArticleCell reports2013
CAST-ChIP maps cell-type-specific chromatin states in the Drosophila central nervous system.
Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- The Tip60 acetylome is a hallmark of the proliferative state in Drosophila.Nucleic acids research · 2025Article
- Experience-dependent Tip60 nucleocytoplasmic transport is regulated by its NLS/NES sequences for neuroplasticity gene control.Molecular and cellular neurosciences · 2023Article
- Chromatin Preparation and Chromatin Immunoprecipitation from Drosophila Heads.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions.Scientific reports · 2022Article
- Article
- Flying Together:International journal of molecular sciences · 2020Review
- Studying alcohol use disorder using Drosophila melanogaster in the era of 'Big Data'.Behavioral and brain functions : BBF · 2019Review
- The sulfite oxidase Shopper controls neuronal activity by regulating glutamate homeostasis in Drosophila ensheathing glia.Nature communications · 2018Article
- DamID profiling of dynamic Polycomb-binding sites in Drosophila imaginal disc development and tumorigenesis.Epigenetics & chromatin · 2018Article
- Systematic genetic interaction studies identify histone demethylase Utx as potential target for ameliorating Huntington's disease.Human molecular genetics · 2018Article
- Production of Small Noncoding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster.Genetics · 2016Article
- Article
- Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing.Nature protocols · 2016Article
- Genome-Wide Approaches to Drosophila Heart Development.Journal of cardiovascular development and disease · 2016Article
- Epigenetics and therapeutic targets mediating neuroprotection.Brain research · 2015Review
- Cell death in development: Signaling pathways and core mechanisms.Seminars in cell & developmental biology · 2015Review
- The female gametophyte: an emerging model for cell type-specific systems biology in plant development.Frontiers in plant science · 2015Review
- Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner.Advances in genetics · 2015Review
- High-resolution digital profiling of the epigenome.Nature reviews. Genetics · 2014Review
- Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
Chromatin organization and gene activity are responsive to developmental and environmental cues. Although many genes are transcribed throughout development and across cell types, much of gene regulation is highly cell-type specific. To readily track chromatin features at the resolution of cell types within complex tissues, we developed and validated chromatin affinity purification from specific cell types by chromatin immunoprecipitation (CAST-ChIP), a broadly applicable biochemical procedure. RNA polymerase II (Pol II) CAST-ChIP identifies ~1,500 neuronal and glia-specific genes in differentiated cells within the adult Drosophila brain. In contrast, the histone H2A.Z is distributed similarly across cell types and throughout development, marking cell-type-invariant Pol II-bound regions. Our study identifies H2A.Z as an active chromatin signature that is refractory to changes across cell fates. Thus, CAST-ChIP powerfully identifies cell-type-specific as well as cell-type-invariant chromatin states, enabling the systematic dissection of chromatin structure and gene regulation within complex tissues such as the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.