ArticleBiophysical journal2013
Elementary functional properties of single HCN2 channels.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 32 citations in OpenAlex.
- Structure mirroring function: What's the 'matter' with the funny current?The Journal of physiology · 2026Review
- HCN1 is a primary HCN Pacemaker Channel in Neurons.Nature communications · 2026Article
- HCN4 channels sense temperature and determine heart rate responses to heat.Nature communications · 2025Article
- Subunit-specific conductance of single homomeric and heteromeric HCN pacemaker channels at femtosiemens resolution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structural basis for hyperpolarization-dependent opening of human HCN1 channel.Nature communications · 2024Article
- Missing validation of key features in HCN single-channel recordings.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural Basis for Hyperpolarization-dependent Opening of the Human HCN1 Channel.bioRxiv : the preprint server for biology · 2023Article
- Kinetic fingerprinting of metabotropic glutamate receptors.Communications biology · 2023Article
- Weak Cation Selectivity in HCN Channels Results From KFunction (Oxford, England) · 2022Article
- Gating movements and ion permeation in HCN4 pacemaker channels.Molecular cell · 2021Article
- Allosteric signaling in C-linker and cyclic nucleotide-binding domain of HCN2 channels.Biophysical journal · 2021Article
- HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons.eLife · 2019Article
- Stepwise activation of a class C GPCR begins with millisecond dimer rearrangement.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- All four subunits of HCN2 channels contribute to the activation gating in an additive but intricate manner.The Journal of general physiology · 2018Article
- Activation gating in HCN2 channels.PLoS computational biology · 2018Article
- Patch-clamp fluorometry-based channel counting to determine HCN channel conductance.The Journal of general physiology · 2016Article
- Nicotine-Mediated ADP to Spike Transition: Double Spiking in Septal Neurons.The Journal of membrane biology · 2016Article
- Conformational Flip of Nonactivated HCN2 Channel Subunits Evoked by Cyclic Nucleotides.Biophysical journal · 2015Article
- NaV1.5 sodium channel window currents contribute to spontaneous firing in olfactory sensory neurons.Journal of neurophysiology · 2014Article
- Family of prokaryote cyclic nucleotide-modulated ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels are tetramers that evoke rhythmic electrical activity in specialized neurons and cardiac cells. These channels are activated by hyperpolarizing voltage, and the second messenger cAMP can further enhance the activation. Despite the physiological importance of HCN channels, their elementary functional properties are still unclear. In this study, we expressed homotetrameric HCN2 channels in Xenopus oocytes and performed single-channel experiments in patches containing either one or multiple channels. We show that the single-channel conductance is as low as 1.67 pS and that channel activation is a one-step process. We also observed that the time between the hyperpolarizing stimulus and the first channel opening, the first latency, determines the activation process alone. Notably, at maximum hyperpolarization, saturating cAMP drives the channel to open for unusually long periods. In particular, at maximum activation by hyperpolarization and saturating cAMP, the open probability approaches unity. In contrast to other reports, no evidence of interchannel cooperativity was observed. In conclusion, single HCN2 channels operate only with an exceptionally low conductance, and both activating stimuli, voltage and cAMP, exclusively control the open probability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.