ArticleACS nano2013
Adhesion maturation of neutrophils on nanoscopically presented platelet glycoprotein Ibα.
Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Recent Insights into NIR-Light-Responsive Materials for Photothermal Cell Treatments.Nanomaterials (Basel, Switzerland) · 2022Review
- MD Simulations on a Well-Built Docking Model Reveal Fine Mechanical Stability and Force-Dependent Dissociation of Mac-1/GPIbα Complex.Frontiers in molecular biosciences · 2021Article
- Modulating Tumor Cell Functions by Tunable Nanopatterned Ligand Presentation.Nanomaterials (Basel, Switzerland) · 2020Review
- Novel Stenotic Microchannels to Study Thrombus Formation in Shear Gradients: Influence of Shear Forces and Human Platelet-Related Factors.International journal of molecular sciences · 2019Article
- Immunoregulation of macrophages by dynamic ligand presentation via ligand-cation coordination.Nature communications · 2019Article
- Serum and Serum Albumin InhibitFrontiers in immunology · 2019Article
- Blue and Long-Wave Ultraviolet Light InduceFrontiers in immunology · 2019Article
- Effect of Adhesion and Substrate Elasticity on Neutrophil Extracellular Trap Formation.Frontiers in immunology · 2019Article
- Chromatin swelling drives neutrophil extracellular trap release.Nature communications · 2018Article
- Proteolytic processing of platelet receptors.Research and practice in thrombosis and haemostasis · 2018Article
- Nanoscale Tuning of VCAM-1 Determines VLA-4-Dependent Melanoma Cell Plasticity on RGD Motifs.Molecular cancer research : MCR · 2018Article
- Tuning Selectivity of Fluorescent Carbon Nanotube-Based Neurotransmitter Sensors.Sensors (Basel, Switzerland) · 2017Article
- Review
- Surface Patterning of Gold Nanoparticles on PEG-Based Hydrogels to Control Cell Adhesion.Polymers · 2017Article
- High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Application of synthetic biology approaches for understanding encounters between cells and their microenvironment.Cell adhesion & migration · 2016Article
- Structural basis for the specific inhibition of glycoprotein Ibα shedding by an inhibitory antibody.Scientific reports · 2016Article
- Effects of shear on P-selectin deposition in microfluidic channels.Biomicrofluidics · 2016Article
- Nanomaterials, inflammation, and tissue engineering.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Neutrophilic granulocytes play a fundamental role in cardiovascular disease. They interact with platelet aggregates via the integrin Mac-1 and the platelet receptor glycoprotein Ibα (GPIbα). In vivo, GPIbα presentation is highly variable under different physiological and pathophysiological conditions. Here, we quantitatively determined the conditions for neutrophil adhesion in a biomimetic in vitro system, which allowed precise adjustment of the spacings between human GPIbα presented on the nanoscale from 60 to 200 nm. Unlike most conventional nanopatterning approaches, this method provided control over the local receptor density (spacing) rather than just the global receptor density. Under physiological flow conditions, neutrophils required a minimum spacing of GPIbα molecules to successfully adhere. In contrast, under low-flow conditions, neutrophils adhered on all tested spacings with subtle but nonlinear differences in cell response, including spreading area, spreading kinetics, adhesion maturation, and mobility. Surprisingly, Mac-1-dependent neutrophil adhesion was very robust to GPIbα density variations up to 1 order of magnitude. This complex response map indicates that neutrophil adhesion under flow and adhesion maturation are differentially regulated by GPIbα density. Our study reveals how Mac-1/GPIbα interactions govern cell adhesion and how neutrophils process the number of available surface receptors on the nanoscale. In the future, such in vitro studies can be useful to determine optimum therapeutic ranges for targeting this interaction.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.