ArticleArteriosclerosis, thrombosis, and vascular biology2013
Novel family of insect salivary inhibitors blocks contact pathway activation by binding to polyphosphate, heparin, and dextran sulfate.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Factor XII in Thrombosis and Thromboinflammation: From Molecular Biology to Clinical Translation.International journal of molecular sciences · 2026Review
- An Update on Polyphosphate In Vivo Activities.Biomolecules · 2024Review
- An Overview of D7 Protein Structure and Physiological Roles in Blood-Feeding Nematocera.Biology · 2022Review
- An overview of the sand fly salivary proteins in vaccine development against leishmaniases.Iranian journal of microbiology · 2022Review
- Engineering a vector-based pan-Leishmania vaccine for humans: proof of principle.Scientific reports · 2020Article
- RNA-sequencing of the Nyssomyia neivai sialome: a sand fly-vector from a Brazilian endemic area for tegumentary leishmaniasis and pemphigus foliaceus.Scientific reports · 2020Article
- Kinins and Their Receptors in Infectious Diseases.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Immunity to vector saliva is compromised by short sand fly seasons in endemic regions with temperate climates.Scientific reports · 2020Article
- Functional and structural similarities of D7 proteins in the independently-evolved salivary secretions of sand flies and mosquitoes.Scientific reports · 2019Article
- Polyphosphate as a Target for Interference With Inflammation and Thrombosis.Frontiers in medicine · 2019Review
- Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.PLoS neglected tropical diseases · 2017Review
- Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.PLoS neglected tropical diseases · 2017Article
- Mast Cell Coupling to the Kallikrein-Kinin System Fuels Intracardiac Parasitism and Worsens Heart Pathology in Experimental Chagas Disease.Frontiers in immunology · 2017Article
- Structure and Function of FS50, a salivary protein from the flea Xenopsylla cheopis that blocks the sodium channel NaScientific reports · 2016Article
- Neutralizing blood-borne polyphosphate in vivo provides safe thromboprotection.Nature communications · 2016Article
- Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica.PLoS neglected tropical diseases · 2016Article
- Differential expression profiles of the salivary proteins SP15 and SP44 from Phlebotomus papatasi.Parasites & vectors · 2016Article
- The polyphosphate-factor XII pathway drives coagulation in prostate cancer-associated thrombosis.Blood · 2015Article
- Impact of Insect Salivary Proteins in Blood Feeding, Host Immunity, Disease, and in the Development of Biomarkers for Vector Exposure.Current opinion in insect science · 2015Article
- What's behind a sand fly bite? The profound effect of sand fly saliva on host hemostasis, inflammation and immunity.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2014Review
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
objectivePolyphosphate and heparin are anionic polymers released by activated mast cells and platelets that are known to stimulate the contact pathway of coagulation. These polymers promote both the autoactivation of factor XII and the assembly of complexes containing factor XI, prekallikrein, and high-molecular-weight kininogen. We are searching for salivary proteins from blood-feeding insects that counteract the effect of procoagulant and proinflammatory factors in the host, including elements of the contact pathway. APPROACH AND
resultsHere, we evaluate the ability of the sand fly salivary proteins, PdSP15a and PdSP15b, to inhibit the contact pathway by disrupting binding of its components to anionic polymers. We attempt to demonstrate binding of the proteins to polyphosphate, heparin, and dextran sulfate. We also evaluate the effect of this binding on contact pathway reactions. We also set out to determine the x-ray crystal structure of PdSP15b and examine the determinants of relevant molecular interactions. Both proteins bind polyphosphate, heparin, and dextran sulfate with high affinity. Through this mechanism they inhibit the autoactivation of factor XII and factor XI, the reciprocal activation of factor XII and prekallikrein, the activation of factor XI by thrombin and factor XIIa, the cleavage of high-molecular-weight kininogen in plasma, and plasma extravasation induced by polyphosphate. The crystal structure of PdSP15b contains an amphipathic helix studded with basic side chains that forms the likely interaction surface.
conclusionsThe results of these studies indicate that the binding of anionic polymers by salivary proteins is used by blood feeders as an antihemostatic/anti-inflammatory mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.