ArticleCell death & disease2013
P2X7 purinoceptors contribute to the death of Schwann cells transplanted into the spinal cord.
Article in Cell death & disease, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Enteric glia and purinergic signaling in health and disease.Neuropharmacology · 2025Review
- Activation of P2X7R Inhibits Proliferation and Promotes the Migration and Differentiation of Schwann Cells.Molecular neurobiology · 2025Article
- P2X7 receptor antagonists modulate experimental autoimmune neuritis via regulation of NLRP3 inflammasome activation and Th17 and Th1 cell differentiation.Journal of neuroinflammation · 2024Article
- Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses.Journal of neuroinflammation · 2022Article
- M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells.Cell death discovery · 2019Article
- Intraneural Injection of ATP Stimulates Regeneration of Primary Sensory Axons in the Spinal Cord.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Variations in Cellular Responses of Mouse T Cells to Adenosine-5'-Triphosphate Stimulation Do Not Depend on P2X7 Receptor Expression Levels but on Their Activation and Differentiation Stage.Frontiers in immunology · 2018Article
- Connexin43 Hemichannels in Satellite Glial Cells, Can They Influence Sensory Neuron Activity?Frontiers in molecular neuroscience · 2017Review
- Article
- Structure-based identification and characterisation of structurally novel human P2X7 receptor antagonists.Biochemical pharmacology · 2016Article
- The P2X7 receptor regulates cell survival, migration and invasion of pancreatic ductal adenocarcinoma cells.Molecular cancer · 2015Article
- The HMGB1 signaling pathway activates the inflammatory response in Schwann cells.Neural regeneration research · 2015Article
- Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury.Purinergic signalling · 2015Article
- The role of purinergic receptors in stem cell differentiation.Computational and structural biotechnology journal · 2015Review
- Adipose derived stem cells and nerve regeneration.Neural regeneration research · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The potential to use Schwann cells (SCs) in neural repair for patients suffering from neurotrauma and neurodegenerative diseases is well recognized. However, significant cell death after transplantation hinders the clinical translation of SC-based therapies. Various factors may contribute to the death of transplanted cells. It is known that prolonged activation of P2X7 purinoceptors (P2X7R) can lead to death of certain types of cells. In this study, we show that rat SCs express P2X7R and exposure of cultured SCs to high concentrations of ATP (3-5 mM) or a P2X7R agonist, 2'(3')-O-(4-benzoylbenzoyl)ATP (BzATP) induced significant cell death rapidly. High concentrations of ATP and BzATP increased ethidium uptake by SCs, indicating increased membrane permeability to large molecules, a typical feature of prolonged P2X7R activation. SC death, as well as ethidium uptake, induced by ATP was blocked by an irreversible P2X7R antagonist oxidized ATP (oxATP) or a reversible P2X7R antagonist A438079. oxATP also significantly inhibits the increase of intracellular free calcium induced by minimolar ATP concentrations. Furthermore, ATP did not cause death of SCs isolated from P2X7R-knockout mice. All these results suggest that P2X7R is responsible for ATP-induced SC death in vitro. When rat SCs were treated with oxATP before transplantation into uninjured rat spinal cord, 35% more SCs survived than untreated SCs 1 week after transplantation. Moreover, 58% more SCs isolated from P2X7R-knockout mice survived after being transplanted into rat spinal cord than SCs from wild-type mice. This further confirms that P2X7R is involved in the death of transplanted SCs. These results indicate that targeting P2X7R on SCs could be a potential strategy to improve the survival of transplanted cells. As many other types of cells, including neural stem cells, also express P2X7R, deactivating P2X7R may improve the survival of other types of transplanted cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.