ArticleProteome science2013
Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients.
Article in Proteome science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Cartilage Homeostasis and Osteoarthritis.International journal of molecular sciences · 2022Review
- Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits.PeerJ · 2022Article
- The Follistatin-like Protein 1 Pathway Is Important for Maintaining Healthy Articular Cartilage.ACR open rheumatology · 2020Article
- Differential Secretome Profiling of Human Osteoarthritic Synoviocytes Treated with Biotechnological Unsulfated and Marine Sulfated Chondroitins.International journal of molecular sciences · 2020Article
- Protein Cytl1: its role in chondrogenesis, cartilage homeostasis, and disease.Cellular and molecular life sciences : CMLS · 2019Review
- Fetal articular cartilage regeneration versus adult fibrocartilaginous repair: secretome proteomics unravels molecular mechanisms in an ovine model.Disease models & mechanisms · 2018Article
- Quantitative proteomics analysis of cartilage response to mechanical injury and cytokine treatment.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Pathways to understanding the genomic aetiology of osteoarthritis.Human molecular genetics · 2017Review
- Characterization of the chondrocyte secretome in photoclickable poly(ethylene glycol) hydrogels.Biotechnology and bioengineering · 2017Article
- Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis.Scientific reports · 2017Article
- Article
- Functional genomics in osteoarthritis: Past, present, and future.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- Aging-related inflammation in osteoarthritis.Osteoarthritis and cartilage · 2015Review
- Comparative genomic profiling of synovium versus skin lesions in psoriatic arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2015Article
- Subchondral bone proteomics in osteoarthritis: Current status and perspectives.Journal of orthopaedic translation · 2015Review
- Quantitative proteomic analysis of single or fractionated radiation-induced proteins in human breast cancer MDA-MB-231 cells.Cell & bioscience · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoarthritis (OA) is a destructive joint disease and there are no known biomarkers available for an early diagnosis. To identify potential disease biomarkers and gain further insight into the disease mechanisms of OA we applied quantitative proteomics with SILAC technology on the secretomes from chondrocytes of OA knees, designated as high Mankin (HM) scored secretome. A quantitative comparison was made between the secretomes of the medial and lateral femur condyle chondrocytes in the same knee since the medial femur condyle is usually more affected in OA than the lateral condyle, which was confirmed by Mankin scoring. The medial/lateral comparison was also made on the secretomes from chondrocytes taken from one individual with no clinically apparent joint-disease, designated as low Mankin (LM) scored secretome.
resultsWe identified 825 proteins in the HM secretome and 69 of these showed differential expression when comparing the medial and lateral femoral compartment. The LM scored femoral condyle showed early signs of OA in the medial compartment as assessed by Mankin score. We here report the identification and relative quantification of several proteins of interest for the OA disease mechanism e.g. CYTL1, DMD and STAB1 together with putative early disease markers e.g. TIMP1, PPP2CA and B2M.
conclusionsThe present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms.
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Registered trials
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