ArticleThe Journal of biological chemistry2013
A key role for Plasmodium subtilisin-like SUB1 protease in egress of malaria parasites from host hepatocytes.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Plasmepsins as Antimalarial Drug Targets-Then, Now, and the Future.Medicinal research reviews · 2026Review
- Accessing invasion-competentScience advances · 2026Article
- Accessing invasion-competent Plasmodium vivax merozoites reveals antibody-dependent NK cell activation.Research square · 2026Article
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- Peptidic Boronic AcidJournal of medicinal chemistry · 2024Article
- Post-Translational Modifications of Proteins of Malaria Parasites during the Life Cycle.International journal of molecular sciences · 2024Review
- Prodomain-driven enzyme dimerization: a pH-dependent autoinhibition mechanism that controlsmBio · 2024Article
- Peptidic boronic acids are potent cell-permeable inhibitors of the malaria parasite egress serine protease SUB1.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Malaria parasite egress at a glance.Journal of cell science · 2021Review
- A Plasmodium cysteine protease required for efficient transition from the liver infection stage.PLoS pathogens · 2020Article
- Characteristic features of the SERA multigene family in the malaria parasite.Parasites & vectors · 2020Review
- Article
- A malaria parasite subtilisin propeptide-like protein is a potent inhibitor of the egress protease SUB1.The Biochemical journal · 2020Article
- Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development.Malaria journal · 2019Article
- The Plasmodium berghei serine protease PbSUB1 plays an important role in male gamete egress.Cellular microbiology · 2019Article
- PKAc is not required for the preerythrocytic stages ofLife science alliance · 2019Article
- Engineered Livers for Infectious Diseases.Cellular and molecular gastroenterology and hepatology · 2018Review
- A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress.Science (New York, N.Y.) · 2017Article
- Plasmodium berghei PIMMS2 Promotes Ookinete Invasion of the Anopheles gambiae Mosquito Midgut.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In their mammalian host, Plasmodium parasites have two obligatory intracellular development phases, first in hepatocytes and subsequently in erythrocytes. Both involve an orchestrated process of invasion into and egress from host cells. The Plasmodium SUB1 protease plays a dual role at the blood stage by enabling egress of the progeny merozoites from the infected erythrocyte and priming merozoites for subsequent erythrocyte invasion. Here, using conditional mutagenesis in P. berghei, we show that SUB1 plays an essential role at the hepatic stage. Stage-specific sub1 invalidation during prehepatocytic development showed that SUB1-deficient parasites failed to rupture the parasitophorous vacuole membrane and to egress from hepatocytes. Furthermore, mechanically released parasites were not adequately primed and failed to establish a blood stage infection in vivo. The critical involvement of SUB1 in both pre-erythrocytic and erythrocytic developmental phases qualifies SUB1 as an attractive multistage target for prophylactic and therapeutic anti-Plasmodium intervention strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.