ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Zebrafish embryonic neurons transport messenger RNA to axons and growth cones in vivo.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Characterization of Transgenic Lines Labeling Reticulospinal Neurons in Larval Zebrafish.eNeuro · 2025Article
- Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration.eLife · 2023Article
- Quantitative assessment of near-infrared fluorescent proteins.Nature methods · 2023Article
- Bridging the Gap: The Importance of TUBA1A α-Tubulin in Forming Midline Commissures.Frontiers in cell and developmental biology · 2021Article
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Mechanisms of Axon Elongation Following CNS Injury: What Is Happening at the Axon Tip?Frontiers in cellular neuroscience · 2020Article
- AnBiology open · 2018Article
- BMP- and neuropilin 1-mediated motor axon navigation relies on spastin alternative translation.Development (Cambridge, England) · 2018Article
- TheJournal of developmental biology · 2017Article
- The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo.PloS one · 2015Article
- Deregulated expression of cytoskeleton related genes in the spinal cord and sciatic nerve of presymptomatic SOD1(G93A) Amyotrophic Lateral Sclerosis mouse model.Frontiers in cellular neuroscience · 2014Article
- A Role for Two-Pore Channel Type 2 (TPC2)-Mediated Regulation of Membrane Contact Sites During Zebrafish Notochord Biogenesis?Contact (Thousand Oaks (Ventura County, Calif.))Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although mRNA was once thought to be excluded from the axonal compartment, the existence of protein synthesis in growing or regenerating axons in culture is now generally accepted. However, its extent and functional importance remain a subject of intense investigation. Furthermore, unambiguous evidence of mRNA axonal transport and local translation in vivo, in the context of a whole developing organism is still lacking. Here, we provide direct evidence of the presence of mRNAs of the tubb5, nefma, and stmnb2 genes in several types of axons in the developing zebrafish (Danio rerio) embryo, with frequent accumulation at the growth cone. We further show that axonal localization of mRNA is a specific property of a subset of genes, as mRNAs of the huc and neurod genes, abundantly expressed in neurons, were not found in axons. We set up a reporter system in which the 3' untranslated region (UTR) of candidate mRNA, fused to a fluorescent protein coding sequence, was expressed in isolated neurons of the zebrafish embryo. Using this reporter, we identified in the 3'UTR of tubb5 mRNA a motif necessary and sufficient for axonal localization. Our work thus establishes the zebrafish as a model system to study axonal transport in a whole developing vertebrate organism, provides an experimental frame to assay this transport in vivo and to study its mechanisms, and identifies a new zipcode involved in axonal mRNA localization.
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