ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014
Overexpressing sonic hedgehog peptide restores periosteal bone formation in a murine bone allograft transplantation model.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Sonic hedgehog reduces alveolar bone resorption by triggering the coupling of type H vessel formation and osteogenesis in a periodontitis model.BMC oral health · 2026Article
- Hedgehog Signalling in Osteogenesis and Bone Metabolism: Molecular Mechanisms, Regulatory Networks and Implications for Skeletal Disease.Journal of cellular and molecular medicine · 2025Review
- Signaling Pathways Driving MSC Osteogenesis: Mechanisms, Regulation, and Translational Applications.International journal of molecular sciences · 2025Review
- Review
- Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration.Current osteoporosis reports · 2022Review
- On the horizon: Hedgehog signaling to heal broken bones.Bone research · 2022Review
- A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.International journal of biological sciences · 2022Article
- Research progress on the hedgehog signalling pathway in regulating bone formation and homeostasis.Cell proliferation · 2022Review
- The periosteum: a simple tissue with many faces, with special reference to the antler-lineage periostea.Biology direct · 2021Review
- Exosomes from miRNA-378-modified adipose-derived stem cells prevent glucocorticoid-induced osteonecrosis of the femoral head by enhancing angiogenesis and osteogenesis via targeting miR-378 negatively regulated suppressor of fused (Sufu).Stem cell research & therapy · 2021Article
- Periosteum Mimetic Coating on Structural Bone AllograftsACS biomaterials science & engineering · 2020Article
- Sox9+ messenger cells orchestrate large-scale skeletal regeneration in the mammalian rib.eLife · 2019Article
- Layer-by-layer nanofiber-enabled engineering of biomimetic periosteum for bone repair and reconstruction.Biomaterials · 2018Article
- Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds.Journal of dental research · 2018Article
- Article
- Lumbar intervertebral disc allograft transplantation: the revascularisation pattern.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2018Article
- Epiphyseal bone formation occurs via thyroid hormone regulation of chondrocyte to osteoblast transdifferentiation.Scientific reports · 2017Article
- Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration.Development (Cambridge, England) · 2016Article
- Lessons on skeletal cell plasticity from studying jawbone regeneration in zebrafish.BoneKEy reports · 2016Review
- Emulating native periosteum cell population and subsequent paracrine factor production to promote tissue engineered periosteum-mediated allograft healing.Biomaterials · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although activation of hedgehog (Hh) signaling has been shown to induce osteogenic differentiation in vitro and bone formation in vivo, the underlying mechanisms and the potential use of Hh-activated mesenchymal progenitors in bone defect repair remain elusive. In this study, we demonstrated that implantation of periosteal-derived mesenchymal progenitor cells (PDMPCs) that overexpressed an N-terminal sonic hedgehog peptide (ShhN) via an adenoviral vector (Ad-ShhN) restored periosteal bone collar formation in a 4-mm segmental bone allograft model in immunodeficient mice. Ad-ShhN enhanced donor cell survival and microvessel formation in collagen scaffold at 2 weeks after surgery and induced donor cell-dependent bone formation at 6 weeks after surgery. Fluorescence-activated cell sorting analysis further showed that Ad-ShhN-PDMPC-seeded scaffold contained a twofold more CD45(-)Sca-1(+)CD34(+)VEGFR2(+) endothelial progenitors than Ad-LacZ-PDMPC-seeded scaffold at day 7 after surgery. Ad-ShhN-transduced PDMPCs induced a 1.8-fold more CD31(+) microvessel formation than Ad-LacZ-transduced PDMPCs in a coculture of endothelial progenitors and PDMPCs. Taken together, our data show that overexpression of ShhN in mesenchymal progenitors improves bone defect reconstruction by enhancing donor progenitor cell survival, differentiation, and scaffold revascularization at the site of compromised periosteum. Hh agonist-based therapy, therefore, merits further investigation in tissue engineering-based applications aimed at enhancing bone defect repair and reconstruction.
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