ArticleScientific reports2013
Characterization of GM events by insert knowledge adapted re-sequencing approaches.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.BMC biotechnology · 2026Article
- Deciphering the complex molecular architecture of the genetically modified soybean FG72 through paired-end whole genome sequencing.Food chemistry. Molecular sciences · 2025Article
- Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.Plant cell reports · 2025Article
- Efficient identification of genomic insertions and surrounding regions in two transgenic maize events using third-generation single-molecule nanopore sequencing technology.Scientific reports · 2024Article
- Review of the technology used for structural characterization of the GMO genome using NGS data.Genomics & informatics · 2024Article
- Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants.EFSA journal. European Food Safety Authority · 2024Article
- An integrated strategy involving high-throughput sequencing to characterize an unknown GM wheat event in Canada.Plant biotechnology journal · 2024Article
- Identifying transgene insertions inPeerJ · 2024Article
- Genetically modified soybean lines exhibit less transcriptomic variation compared to natural varieties.GM crops & food · 2023Article
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- Development and evaluation of triple gene transgenic cotton lines expressing three genes (Cry1Ac-Cry2Ab-EPSPS) for lepidopteran insect pests and herbicide tolerance.Scientific reports · 2022Article
- Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.Food chemistry. Molecular sciences · 2022Article
- A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice.Communications biology · 2022Article
- LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.Plant biotechnology journal · 2022Article
- TC-hunter: identification of the insertion site of a transgenic gene within the host genome.BMC genomics · 2022Article
- Whole-genome resequencing using next-generation and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741.BMC genomics · 2021Article
- Influence of transgenesis on genome variability in cucumber lines with aPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Article
- Development of event-specific detection method for identification of insect resistant NIBGE-1601 cotton harboring double gene Cry1Ac-Cry2Ab construct.Scientific reports · 2021Article
- Efficient identification of genomic insertions and flanking regions through whole-genome sequencing in three transgenic soybean events.Transgenic research · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection methods and data from molecular characterization of genetically modified (GM) events are needed by stakeholders of public risk assessors and regulators. Generally, the molecular characteristics of GM events are incomprehensively revealed by current approaches and biased towards detecting transformation vector derived sequences. GM events are classified based on available knowledge of the sequences of vectors and inserts (insert knowledge). Herein we present three insert knowledge-adapted approaches for characterization GM events (TT51-1 and T1c-19 rice as examples) based on paired-end re-sequencing with the advantages of comprehensiveness, accuracy, and automation. The comprehensive molecular characteristics of two rice events were revealed with additional unintended insertions comparing with the results from PCR and Southern blotting. Comprehensive transgene characterization of TT51-1 and T1c-19 is shown to be independent of a priori knowledge of the insert and vector sequences employing the developed approaches. This provides an opportunity to identify and characterize also unknown GM events.
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