ArticleThe ISME journal2014
Purine biosynthesis-deficient Burkholderia mutants are incapable of symbiotic accommodation in the stinkbug.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- InferiorMicrobial genomics · 2024Pooled it
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- Strict gut symbiont specificity in Coreoidea insects governed by interspecies competition within Caballeronia strains.The ISME journal · 2025Article
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- Alteration of lipopolysaccharide O antigen leads to avirulence of gut-colonizingFrontiers in microbiology · 2023Article
- How It All Begins: Bacterial Factors Mediating the Colonization of Invertebrate Hosts by Beneficial Symbionts.Microbiology and molecular biology reviews : MMBR · 2022Review
- Novel metabolic interactions and environmental conditions mediate the boreal peatmoss-cyanobacteria mutualism.The ISME journal · 2022Article
- The Evolution of Fluoroquinolone Resistance inInternational journal of molecular sciences · 2021Article
- Dual oxidase enables insect gut symbiosis by mediating respiratory network formation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Gut Microbiota of the Insect Infraorder Pentatomomorpha (Hemiptera: Heteroptera) for the Light of Ecology and Evolution.Microorganisms · 2021Review
- Multiple Precursor Proteins of Thanatin Isoforms, an Antimicrobial Peptide Associated With the Gut Symbiont ofFrontiers in microbiology · 2021Article
- The Importance of Environmentally Acquired Bacterial Symbionts for the Squash Bug (Frontiers in microbiology · 2021Article
- Article
- Burkholderia insecticola triggers midgut closure in the bean bug Riptortus pedestris to prevent secondary bacterial infections of midgut crypts.The ISME journal · 2020Article
- Inhibition ofPathogens (Basel, Switzerland) · 2020Article
- Host-symbiont specificity determined by microbe-microbe competition in an insect gut.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Human Bile-Mediated Regulation ofJournal of bacteriology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Riptortus-Burkholderia symbiotic system represents a promising experimental model to study the molecular mechanisms involved in insect-bacterium symbiosis due to the availability of genetically manipulated Burkholderia symbiont. Using transposon mutagenesis screening, we found a symbiosis-deficient mutant that was able to colonize the host insect but failed to induce normal development of host's symbiotic organ. The disrupted gene was identified as purL involved in purine biosynthesis. In vitro growth impairment of the purL mutant and its growth dependency on adenine and adenosine confirmed the functional disruption of the purine synthesis gene. The purL mutant also showed defects in biofilm formation, and this defect was not rescued by supplementation of purine derivatives. When inoculated to host insects, the purL mutant was initially able to colonize the symbiotic organ but failed to attain a normal infection density. The low level of infection density of the purL mutant attenuated the development of the host's symbiotic organ at early instar stages and reduced the host's fitness throughout the nymphal stages. Another symbiont mutant-deficient in a purine biosynthesis gene, purM, showed phenotypes similar to those of the purL mutant both in vitro and in vivo, confirming that the purL phenotypes are due to disrupted purine biosynthesis. These results demonstrate that the purine biosynthesis genes of the Burkholderia symbiont are critical for the successful accommodation of symbiont within the host, thereby facilitating the development of the host's symbiotic organ and enhancing the host's fitness values.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.