ArticleJournal of translational medicine2013
Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD).
Article in Journal of translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Sex differences in kidney and lung status in an animal model of brain death.Clinics (Sao Paulo, Brazil) · 2025Article
- Effect of prolactin on cytotoxicity and oxidative stress in ovine ovarian granulosa cells.PeerJ · 2023Article
- Brain stem death induces pro-inflammatory cytokine production and cardiac dysfunction in sheep model.Biomedical journal · 2022Article
- CustodiolInternational journal of molecular sciences · 2021Article
- Immunomodulatory effects of thalidomide in an experimental brain death liver donor model.Scientific reports · 2021Article
- Mesenchymal stem cell-derived conditioned medium protects vascular grafts of brain-dead rats against in vitro ischemia/reperfusion injury.Stem cell research & therapy · 2021Article
- Dietary Glycine Prevents FOLFOX Chemotherapy-Induced Heart Injury: A Colorectal Cancer Liver Metastasis Treatment Model in Rats.Nutrients · 2020Article
- Rapid or Slow Time to Brain Death? Impact on Kidney Graft Injuries in an Allotransplantation Porcine Model.International journal of molecular sciences · 2019Article
- Renal effects of exendin-4 in an animal model of brain death.Molecular biology reports · 2019Article
- Prolonged Duration of Brain Death was Associated with Better Kidney Allograft Function and Survival: A Prospective Cohort Analysis.Annals of transplantation · 2019Article
- A porcine model to study the effect of brain death on kidney genomic responses.Journal of clinical and translational science · 2018Article
- The effects of brain death and ischemia on tolerance induction are organ-specific.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2018Article
- Enhanced protection of the renal vascular endothelium improves early outcome in kidney transplantation: Preclinical investigations in pig and mouse.Scientific reports · 2018Article
- The Crosstalk between ROS and Autophagy in the Field of Transplantation Medicine.Oxidative medicine and cellular longevity · 2017Review
- A standardized model of brain death, donor treatment, and lung transplantation for studies on organ preservation and reconditioning.Intensive care medicine experimental · 2014Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs organ shortage is increasing, the acceptance of marginal donors increases, which might result in poor organ function and patient survival. Mostly, organ damage is caused during brain death (BD), cold ischemic time (CIT) or after reperfusion due to oxidative stress or the induction of apoptosis. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and compare these findings with immunohistochemistry from a BD and living donation (LD) pig model and after cold ischemia time (CIT).
methodsBD was induced in pigs; after 12 h organ retrieval was performed; heart, liver and kidney tissue specimens were collected in the BD (n = 6) and in a LD model (n = 6). PCR analysis for NFKB1, GSS, SOD2, PPAR-alpha, OXSR1, BAX, BCL2L1, and HSP 70.2 was performed and immunohistochemistry used to show apoptosis and nitrosative stress induced cell damage.
resultsIn heart tissue of BD BAX, BCL2L1 and HSP 70.2 increased significantly after CIT. Only SOD2 was over-expressed after CIT in BD liver tissue. In kidney tissue, BCL2L1, NFKB, OXSR1, SOD2 and HSP 70.2 expression was significantly elevated in LD. Immunohistochemistry showed a significant increase in activated Caspase 3 and nitrotyrosine positive cells after CIT in BD in liver and in kidney tissue but not in heart tissue.
conclusionThe up-regulation of protective and apoptotic genes seems to be divergent in the different organs in the BD and LD setting; however, immunohistochemistry revealed more apoptotic and nitrotyrosine positive cells in the BD setting in liver and kidney tissue whereas in heart tissue both BD and LD showed an increase.
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