ArticleMolecular biology of the cell2013
Wnt5a signaling promotes apical and basolateral polarization of single epithelial cells.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Rho activation drives luminal collapse and eversion in epithelial acini.Biophysical journal · 2023Article
- Wnt5A signaling supports antigen processing and CD8 T cell activation.Frontiers in immunology · 2022Article
- Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis.Frontiers in immunology · 2022Article
- FoxL1Cells & development · 2021Article
- Src and Fyn define a new signaling cascade activated by canonical and non-canonical Wnt ligands and required for gene transcription and cell invasion.Cellular and molecular life sciences : CMLS · 2020Article
- Lef1 regulates caveolin expression and caveolin dependent endocytosis, a process necessary for Wnt5a/Ror2 signaling during Xenopus gastrulation.Scientific reports · 2019Article
- Regulation of Rho GTPase activity at the leading edge of migrating cells by p190RhoGAP.Small GTPases · 2019Review
- Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor.Stem cells international · 2019Article
- CK1ε and p120-catenin control Ror2 function in noncanonical Wnt signaling.Molecular oncology · 2018Article
- A Tuba/Cdc42/Par6A complex is required to ensure singularity in apical domain formation during enterocyte polarization.PloS one · 2018Article
- The Dickkopf1-cytoskeleton-associated protein 4 axis creates a novel signalling pathway and may represent a molecular target for cancer therapy.British journal of pharmacology · 2017Review
- An apicobasal gradient of Rac activity determines protrusion form and position.Nature communications · 2017Article
- Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway.Nature communications · 2016Article
- Arl4c expression in colorectal and lung cancers promotes tumorigenesis and may represent a novel therapeutic target.Oncogene · 2015Article
- A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.The EMBO journal · 2014Article
- The interdependence of the Rho GTPases and apicobasal cell polarity.Small GTPases · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single epithelial-derived tumor cells have been shown to induce apical and basolateral (AB) polarity by expression of polarization-related proteins. However, physiological cues and molecular mechanisms for AB polarization of single normal epithelial cells are unclear. When intestinal epithelial cells 6 (IEC6 cells) were seeded on basement membrane proteins (Matrigel), single cells formed an F-actin cap on the upper cell surface, where apical markers accumulated, and a basolateral marker was localized to the rest of the cell surface region, in a Wnt5a signaling-dependent manner. However, these phenotypes were not induced by type I collagen. Rac1 activity in the noncap region was higher than that in the cap region, whereas Rho activity increased toward the cap region. Wnt5a signaling activated and inhibited Rac1 and RhoA, respectively, independently through Tiam1 and p190RhoGAP-A, which formed a tertiary complex with Dishevelled. Furthermore, Wnt5a signaling through Rac1 and RhoA was required for cystogenesis of IEC6 cells. These results suggest that Wnt5a promotes the AB polarization of IEC6 cells through regulation of Rac and Rho activities in a manner dependent on adhesion to specific extracellular matrix proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.