ArticleAnalytical chemistry2013
Profiling deacetylase activities in cell lysates with peptide arrays and SAMDI mass spectrometry.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates.Scientific reports · 2022Article
- Label-Free Screening of SARS-CoV-2 NSP14 Exonuclease Activity Using SAMDI Mass Spectrometry.SLAS discovery : advancing life sciences R & D · 2021Article
- Hydroxamic acid-modified peptide microarrays for profiling isozyme-selective interactions and inhibition of histone deacetylases.Nature communications · 2021Article
- Article
- Profiling Protein Tyrosine Phosphatase Specificity with Self-Assembled Monolayers for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Peptide Arrays.ACS combinatorial science · 2019Article
- Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells.Analytical chemistry · 2019Article
- Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue.ChemMedChem · 2019Article
- High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA.Science advances · 2019Article
- Traceless Immobilization of Analytes for High-Throughput Experiments with SAMDI Mass Spectrometry.Journal of the American Chemical Society · 2018Article
- Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.Nature chemical biology · 2018Article
- Peptide Arrays: Development and Application.Analytical chemistry · 2018Review
- Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities.Methods in enzymology · 2018Article
- Genetically Encoded Fluorescent Probe for Detecting Sirtuins in Living Cells.Journal of the American Chemical Society · 2017Article
- Machine Learning on Signal-to-Noise Ratios Improves Peptide Array Design in SAMDI Mass Spectrometry.Analytical chemistry · 2017Article
- Cellular Assays with a Molecular Endpoint Measured by SAMDI Mass Spectrometry.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- SIRT1 is a critical regulator of K562 cell growth, survival, and differentiation.Experimental cell research · 2016Article
- Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules.Nanoscale · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The development of arrays that can profile molecular activities in cells is important to understanding signaling pathways in normal and pathological settings. While oligonucleotide arrays are now routinely used to profile global gene expression, there is still a lack of tools for profiling enzyme activities in cell lysates. This paper describes the combination of peptide arrays formed on self-assembled monolayers and mass spectrometry to provide a label-free approach for identifying patterns of enzyme activities in cell lysates. The approach is demonstrated by profiling lysine deacetylase (KDAC) activities in cell lysates of the CHRF megakaryocytic (Mk) cell line. Class-specific deacetylase inhibitors were used to show that terminal Mk differentiation of CHRF cells is marked by a pronounced decrease in sirtuin activity and by little change in activity of KDACs 1-11. This work establishes a platform that can be used to identify changes in global activity profiles of cell lysates for a wide variety of enzymatic activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.