ArticlePloS one2013
MicroRNA-212 post-transcriptionally regulates oocyte-specific basic-helix-loop-helix transcription factor, factor in the germline alpha (FIGLA), during bovine early embryogenesis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Primary Ovarian Insufficiency: Molecular Mechanisms and the Role of MicroRNAs.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation.International journal of molecular sciences · 2025Review
- Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development.International journal of nanomedicine · 2025Article
- DNA Methylation Profiling of Ovarian Tissue of Climbing Perch (Anabas testudienus) in Response to Monocrotophos Exposure.Marine biotechnology (New York, N.Y.) · 2023Article
- Epitranscriptome marks detection and localization of RNA modifying proteins in mammalian ovarian follicles.Journal of ovarian research · 2023Article
- Role of miRNAs interference on ovarian functions and premature ovarian failure.Cell communication and signaling : CCS · 2022Review
- Embryonic microRNAs are essential for bovine preimplantation embryo development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence.International journal of molecular sciences · 2020Article
- DNA methylation and miRNA-1296 act in concert to mediate spatiotemporal expression of KPNA7 during bovine oocyte and early embryonic development.BMC developmental biology · 2019Article
- Embryo competence and cryosurvival: Molecular and cellular features.Animal reproduction · 2019Article
- Article
- Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis).Scientific reports · 2016Article
- The Role of Maternal-Effect Genes in Mammalian Development: Are Mammalian Embryos Really an Exception?Stem cell reviews and reports · 2016Review
- MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans.Stem cells international · 2016Review
- Controlled ovarian hyperstimulation induced changes in the expression of circulatory miRNA in bovine follicular fluid and blood plasma.Journal of ovarian research · 2015Article
- MicroRNA in Ovarian Biology and Disease.Cold Spring Harbor perspectives in medicine · 2015Review
- Oocyte ageing and epigenetics.Reproduction (Cambridge, England) · 2015Review
- The Maternal-to-Zygotic Transition During Vertebrate Development: A Model for Reprogramming.Current topics in developmental biology · 2015Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Factor in the germline alpha (FIGLA) is an oocyte-specific basic helix-loop-helix transcription factor essential for primordial follicle formation and expression of many genes required for folliculogenesis, fertilization and early embryonic survival. Here we report the characterization of bovine FIGLA gene and its regulation during early embryogenesis. Bovine FIGLA mRNA expression is restricted to gonads and is detected in fetal ovaries harvested as early as 90 days of gestation. FIGLA mRNA and protein are abundant in germinal vesicle and metaphase II stage oocytes, as well as in embryos from pronuclear to eight-cell stage but barely detectable at morula and blastocyst stages, suggesting that FIGLA might be a maternal effect gene. Recent studies in zebrafish and mice have highlighted the importance of non-coding small RNAs (microRNAs) as key regulatory molecules targeting maternal mRNAs for degradation during embryonic development. We hypothesized that FIGLA, as a maternal transcript, is regulated by microRNAs during early embryogenesis. Computational predictions identified a potential microRNA recognition element (MRE) for miR-212 in the 3' UTR of the bovine FIGLA mRNA. Bovine miR-212 is expressed in oocytes and tends to increase in four-cell and eight-cell stage embryos followed by a decline at morula and blastocyst stages. Transient transfection and reporter assays revealed that miR-212 represses the expression of FIGLA in a MRE dependent manner. In addition, ectopic expression of miR-212 mimic in bovine early embryos dramatically reduced the expression of FIGLA protein. Collectively, our results demonstrate that FIGLA is temporally regulated during bovine early embryogenesis and miR-212 is an important negative regulator of FIGLA during the maternal to zygotic transition in bovine embryos.
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