ArticlePloS one2013
Systematic application of DNA fiber-FISH technique in cotton.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Post-polyploidization centromere evolution in cotton.Nature genetics · 2025Article
- Article
- LINE-1 and SINE-B1 mapping and genome diversification inLife science alliance · 2022Article
- Localization and characterization of Citrus centromeres by combining half-tetrad analysis and CenH3-associated sequence profiling.Plant cell reports · 2020Article
- Telomere Length Measurement by Molecular Combing.Frontiers in cell and developmental biology · 2020Article
- Article
- Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum.Scientific reports · 2017Article
- Article
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Authors and funding
4 authors.
Funding
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Abstract
Fluorescence in situ hybridization on extended DNA (fiber-FISH) is a powerful tool in high-resolution physical mapping. To introduce this technique into cotton, we developed the technique and tested it by deliberately mapping of telomere and 5S rDNA. Results showed that telomere-length ranged from 0.80 kb to 37.86 kb in three species, G. hirsutum, G. herbaceum and G. arboreum. However, most of the telomeres (>91.0%) were below 10 kb. The length of 5S rDNA was revealed as 964 kb in G. herbaceum whereas, in G. arboreum, it was approximately three times longer (3.1 Mb). A fiber-FISH based immunofluorescence method was also described to assay the DNA methylation. Using this technique, we revealed that both telomere and 5S rDNA were methylated at different levels. In addition, we developed a BAC molecule-based fiber-FISH technique. Using this technique, we can precisely map BAC clones on each other and evaluated the size and location of overlapped regions. The development and application of fiber-FISH technique will facilitate high-resolution physical mapping and further directed sequencing projects for cotton.
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