ArticlePloS one2013
Multiplex microsphere immunoassays for the detection of IgM and IgG to arboviral diseases.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Human Seroprevalence for Dengue, Ross River, and Barmah Forest viruses in Australia and the Pacific: A systematic review spanning seven decades.PLoS neglected tropical diseases · 2022Pooled it
- Powassan and Eastern Equine Encephalitis Virus Seroprevalence in Endemic Areas, United States, 2019-2020.Emerging infectious diseases · 2025Article
- Serological insights from SARS-CoV-2 heterologous prime and boost responses in Thailand.Scientific reports · 2025Article
- Pathogenicity and virulence of chikungunya virus.Virulence · 2024Review
- Dengue: A review of laboratory diagnostics in the vaccine age.Journal of medical microbiology · 2024Review
- Association between seroprevalence of measles virus in monkeys and degree of human-monkey contact in Bangladesh.One health (Amsterdam, Netherlands) · 2023Article
- Article
- Geographical distribution and pathogenesis of ticks and tick-borne viral diseases.Frontiers in microbiology · 2023Review
- Multiplex detection of antibodies to Chikungunya, O'nyong-nyong, Zika, Dengue, West Nile and Usutu viruses in diverse non-human primate species from Cameroon and the Democratic Republic of Congo.PLoS neglected tropical diseases · 2021Article
- Comparison of Zika virus inactivation methods for reagent production and disinfection methods.Journal of virological methods · 2021Article
- Diagnostic Approach for Arboviral Infections in the United States.Journal of clinical microbiology · 2020Review
- Evaluation for Arboviral Infection Among Children Hospitalized in Colorado With Aseptic Meningitis and Encephalitis.The Pediatric infectious disease journal · 2020Article
- Development of a multiplex microsphere immunoassay for the detection of antibodies against highly pathogenic viruses in human and animal serum samples.PLoS neglected tropical diseases · 2020Article
- Improved Detection of Antibodies against SARS-CoV-2 by Microsphere-Based Antibody Assay.International journal of molecular sciences · 2020Article
- Serological Diagnosis of Flavivirus-Associated Human Infections.Diagnostics (Basel, Switzerland) · 2020Review
- Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses.Scientific reports · 2019Article
- Optimisation and standardisation of a multiplex immunoassay of diverseWellcome open research · 2019Article
- Tick-Borne Flaviviruses, with a Focus on Powassan Virus.Clinical microbiology reviews · 2019Review
- Yellow fever in the diagnostics laboratory.Emerging microbes & infections · 2018Review
- Detection of Specific ZIKV IgM in Travelers Using a Multiplexed Flavivirus Microsphere Immunoassay.Viruses · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serodiagnosis of arthropod-borne viruses (arboviruses) at the Division of Vector-Borne Diseases, CDC, employs a combination of individual enzyme-linked immunosorbent assays and microsphere immunoassays (MIAs) to test for IgM and IgG, followed by confirmatory plaque-reduction neutralization tests. Based upon the geographic origin of a sample, it may be tested concurrently for multiple arboviruses, which can be a cumbersome task. The advent of multiplexing represents an opportunity to streamline these types of assays; however, because serologic cross-reactivity of the arboviral antigens often confounds results, it is of interest to employ data analysis methods that address this issue. Here, we constructed 13-virus multiplexed IgM and IgG MIAs that included internal and external controls, based upon the Luminex platform. Results from samples tested using these methods were analyzed using 8 different statistical schemes to identify the best way to classify the data. Geographic batteries were also devised to serve as a more practical diagnostic format, and further samples were tested using the abbreviated multiplexes. Comparative error rates for the classification schemes identified a specific boosting method based on logistic regression "Logitboost" as the classification method of choice. When the data from all samples tested were combined into one set, error rates from the multiplex IgM and IgG MIAs were <5% for all geographic batteries. This work represents both the most comprehensive, validated multiplexing method for arboviruses to date, and also the most systematic attempt to determine the most useful classification method for use with these types of serologic tests.
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