ArticlePloS one2013
Microfabricated polyacrylamide devices for the controlled culture of growing cells and developing organisms.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Article
- Tracking bacteria at high density with FAST, the Feature-Assisted Segmenter/Tracker.PLoS computational biology · 2023Article
- Depletion attractions drive bacterial capture on both non-fouling and adhesive surfaces, enhancing cell orientation.Soft matter · 2022Article
- The Tumor Microenvironment: An Introduction to the Development of Microfluidic Devices.Advances in experimental medicine and biology · 2022Review
- Hydrogels as artificial matrices for cell seeding in microfluidic devices.RSC advances · 2020Review
- Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces.Materials (Basel, Switzerland) · 2020Article
- Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.Nature communications · 2018Article
- High-throughput screening in the C. elegans nervous system.Molecular and cellular neurosciences · 2017Review
- Deep Learning Automates the Quantitative Analysis of Individual Cells in Live-Cell Imaging Experiments.PLoS computational biology · 2016Article
- Long-term time-lapse microscopy of C. elegans post-embryonic development.Nature communications · 2016Article
- Microfluidic Approaches for Manipulating, Imaging, and Screening C. elegans.Micromachines · 2016Review
- Single-Cell Analysis of the Dps Response to Oxidative Stress.Journal of bacteriology · 2016Article
- Article
- Fitness Trade-Offs in Competence Differentiation of Bacillus subtilis.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to spatially confine living cells or small organisms while dynamically controlling their aqueous environment is important for a host of microscopy applications. Here, we show how polyacrylamide layers can be patterned to construct simple microfluidic devices for this purpose. We find that polyacrylamide gels can be molded like PDMS into micron-scale structures that can enclose organisms, while being permeable to liquids, and transparent to allow for microscopic observation. We present a range of chemostat-like devices to observe bacterial and yeast growth, and C. elegans nematode development. The devices can integrate PDMS layers and allow for temporal control of nutrient conditions and the presence of drugs on a minute timescale. We show how spatial confinement of motile C. elegans enables for time-lapse microscopy in a parallel fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.