ArticlePloS one2013
The transcriptional regulator Np20 is the zinc uptake regulator in Pseudomonas aeruginosa.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- A genome-wide screen inJournal of bacteriology · 2026Article
- Cobalt mitigates zinc-starvation effects in Pseudomonas aeruginosa.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- Pseudopaline-mediated zinc uptake byInfection and immunity · 2026Article
- Modelling host-pathogen interactions:Microbiology (Reading, England) · 2025Article
- Article
- Zinc homeostasis in Pseudomonas.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Investigation of the Role of a Zinc Uptake Regulator (Zur) in the Virulence ofInternational journal of molecular sciences · 2023Article
- A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa.BMC microbiology · 2023Article
- Virulence regulation of ZnFrontiers in veterinary science · 2023Article
- Identification of the Preferred DNA-Binding Sequence and Transcription Regulatory Network for the Thermophilic Zinc Uptake Regulator TTHA1292.Journal of bacteriology · 2022Article
- New insights about the EptA protein and its correlation with theCurrent research in microbial sciences · 2021Article
- The Human Innate Immune Protein Calprotectin Elicits a Multimetal Starvation Response in Pseudomonas aeruginosa.Microbiology spectrum · 2021Article
- Structural basis for zinc-induced activation of a zinc uptake transcriptional regulator.Nucleic acids research · 2021Article
- Calprotectin-Mediated Zinc Chelation Inhibits Pseudomonas aeruginosa Protease Activity in Cystic Fibrosis Sputum.Journal of bacteriology · 2021Article
- Identification and Characterization ofJournal of bacteriology · 2021Article
- Zur: Zinc-Sensing Transcriptional Regulator in a Diverse Set of Bacterial Species.Pathogens (Basel, Switzerland) · 2021Review
- Strategies for Zinc Uptake inFrontiers in microbiology · 2021Review
- Global Analysis of the Zinc Homeostasis Network inFrontiers in microbiology · 2021Article
- Availability of Zinc Impacts Interactions between Streptococcus sanguinis and Pseudomonas aeruginosa in Coculture.Journal of bacteriology · 2020Article
- The CzcCBA Efflux System Requires the CadA P-Type ATPase for Timely Expression Upon Zinc Excess inFrontiers in microbiology · 2020Article
Corrections and comments
- Erratum issuedFarrow, John Matthew 4th [corrected to Farrow, John M 3rd]
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Zinc is essential for all bacteria, but excess amounts of the metal can have toxic effects. To address this, bacteria have developed tightly regulated zinc uptake systems, such as the ZnuABC zinc transporter which is regulated by the Fur-like zinc uptake regulator (Zur). In Pseudomonas aeruginosa, a Zur protein has yet to be identified experimentally, however, sequence alignment revealed that the zinc-responsive transcriptional regulator Np20, encoded by np20 (PA5499), shares high sequence identity with Zur found in other bacteria. In this study, we set out to determine whether Np20 was functioning as Zur in P. aeruginosa. Using RT-PCR, we determined that np20 (hereafter known as zur) formed a polycistronic operon with znuC and znuB. Mutant strains, lacking the putative znuA, znuB, or znuC genes were found to grow poorly in zinc deplete conditions as compared to wild-type strain PAO1. Intracellular zinc concentrations in strain PAO-Zur (Δzur) were found to be higher than those for strain PAO1, further implicating the zur as the zinc uptake regulator. Reporter gene fusions and real time RT-PCR revealed that transcription of znuA was repressed in a zinc-dependent manner in strain PAO1, however zinc-dependent transcriptional repression was alleviated in strain PAO-Zur, suggesting that the P. aeruginosa Zur homolog (ZurPA) directly regulates expression of znuA. Electrophoretic mobility shift assays also revealed that recombinant ZurPA specifically binds to the promoter region of znuA and does not bind in the presence of the zinc chelator N,N',N-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN). Taken together, these data support the notion that Np20 is the P. aeruginosa Zur, which regulates the transcription of the genes encoding the high affinity ZnuABC zinc transport system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.