ArticlePloS one2013
The small GTPase RhoB regulates TNFα signaling in endothelial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Non-canonical and constitutive activation of small GTPases: more than an exception to the rule?The Biochemical journal · 2026Review
- The Molecular Machinery of Synaptic Plasticity and Its Potential Role in the Aetiology of Schizophrenia.Molecular neurobiology · 2026Review
- Immune cells with senescence-related transcriptional signatures orchestrate the inflammatory continuum in osteoarthritis synovium: a single-cell and machine learning study.Frontiers in immunology · 2026Article
- The effect of circulating iron on barrier integrity of primary human endothelial cells.Scientific reports · 2023Article
- Article
- Inflammatory Immune Cytokine TNF-α Modulates Ezrin Protein ActivationFrontiers in pharmacology · 2021Article
- Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.Frontiers in cell and developmental biology · 2021Article
- RhoB is regulated by hypoxia and modulates metastasis in breast cancer.Cancer reports (Hoboken, N.J.) · 2020Article
- RhoA, RhoB and RhoC differentially regulate endothelial barrier function.Small GTPases · 2019Article
- CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity.Scientific reports · 2019Article
- The RhoB small GTPase in physiology and disease.Small GTPases · 2018Review
- Parallels between single cell migration and barrier formation: The case of RhoB and Rac1 trafficking.Small GTPases · 2018Review
- The Cullin-3-Rbx1-KCTD10 complex controls endothelial barrier function via K63 ubiquitination of RhoB.The Journal of cell biology · 2018Article
- RhoB regulates the function of macrophages in the hypoxia-induced inflammatory response.Cellular & molecular immunology · 2017Article
- RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border.The Journal of cell biology · 2016Article
- Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells.Scientific reports · 2016Article
- Article
- Toward understanding RhoGTPase specificity: structure, function and local activation.Small GTPases · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inflammatory response of endothelial cells triggered by cytokines such as TNFα and IL1β plays a pivotal role in innate immunity. Upon pro-inflammatory cytokine stimulation, endothelial cells produce chemokines and cytokines that attract and activate leukocytes, and express high levels of leukocyte adhesion molecules. This process is mediated by intracellular signaling cascades triggered by activation of e.g. the TNFα receptor (TNFR) that lead to the activation of the NFκB transcription factor and of MAP kinases, which in turn activate inflammatory gene transcription. We found that the small GTPase RhoB was strongly and rapidly upregulated in primary human endothelial cells by TNFα, IL1β and LPS. We subsequently investigated the role of RhoB in the regulation of TNFR signaling in endothelial cells by silencing RhoB expression with siRNA. We provide evidence that the TNFα-induced activation of p38 MAP kinase is strongly dependent on RhoB, but not on RhoA, while JNK activation is regulated by both RhoB and RhoA. Consistent with the important role of p38 MAP kinase in inflammation, we demonstrate that loss of RhoB impairs TNFα-induced ICAM-1 expression and reduces cell production of IL6 and IL8. In addition, we show that RhoB silencing alters the intracellular traffic of TNFα after endocytosis. Since RhoB is a known regulator of the intracellular traffic of membrane receptors, our data suggest that RhoB controls TNFα signaling through the regulation of the TNFR traffic.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.