ArticlePloS one2013
High performance of histidine-rich protein 2 based rapid diagnostic tests in French Guiana are explained by the absence of pfhrp2 gene deletion in P. falciparum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 5 of them syntheses that pooled it.
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27 citing papers in PubMed, 5 syntheses or guidelines pooled it, 46 citations in OpenAlex.
- Impact of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions on malaria control worldwide: a systematic review and meta-analysis.Malaria journal · 2021Pooled it
- Rapid diagnostic tests for Plasmodium vivax malaria in endemic countries.The Cochrane database of systematic reviews · 2020Pooled it
- Prevalence ofBulletin of the World Health Organization · 2020Pooled it
- Prevalence of Plasmodium falciparum field isolates with deletions in histidine-rich protein 2 and 3 genes in context with sub-Saharan Africa and India: a systematic review and meta-analysis.Malaria journal · 2020Pooled it
- Rapid diagnostic tests for diagnosing uncomplicated non-falciparum or Plasmodium vivax malaria in endemic countries.The Cochrane database of systematic reviews · 2014Pooled it
- Are pfhrp2 and pfhrp3 Deletions a Concern in Latin America? A Critical Review.Pathogens (Basel, Switzerland) · 2026Review
- Very low prevalence of Plasmodium falciparum histidine-rich protein 2 (pfhrp2) gene deletion in the Brazil, Venezuela, and Guyana tri-border.Scientific reports · 2025Article
- High Frequency of Deletions in theTropical medicine and infectious disease · 2024Article
- Widespread pfhrp2/3 deletions and HRP2-based false-negative results in southern Ethiopia.Malaria journal · 2024Article
- Factors Affecting the Performance of HRP2-Based Malaria Rapid Diagnostic Tests.Tropical medicine and infectious disease · 2022Review
- Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia.Malaria journal · 2021Article
- A Comprehensive Analysis of the Genetic Diversity ofFrontiers in cellular and infection microbiology · 2021Article
- Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania.Malaria journal · 2020Article
- Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice.International journal of environmental research and public health · 2020Article
- Presence of additional Plasmodium vivax malaria in Duffy negative individuals from Southwestern Nigeria.Malaria journal · 2020Article
- Review
- Genetic polymorphism of histidine rich protein 2 in Plasmodium falciparum isolates from different infection sources in Yunnan Province, China.Malaria journal · 2019Article
- Insights into the Performance of SD Bioline Malaria Ag P.f/Pan Rapid Diagnostic Test andThe American journal of tropical medicine and hygiene · 2018Article
- Case Report: A Case ofThe American journal of tropical medicine and hygiene · 2017Article
- Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.PloS one · 2017Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCare for malaria patients in endemic areas has been improved through the increasing use of Rapid Diagnostic Tests (RDTs). Most RDTs target the histidine-rich protein-2 antigen (PfHRP2) to detect P. falciparum, as it is abundant and shows great heat stability. However, their use in South America has been widely questioned following a recent publication that pinpoints the high prevalence of Peruvian field isolates lacking the gene encoding this protein. In the remote rural health centers of French Guiana, RDTs are the main diagnosis tools. Therefore, a study of PfHRP2 RDT performances and pfhrp2 genotyping was conducted to determine whether a replacement of the current pLDH-based kit could be considered.
methodsThe performance study compared the SD Malaria Ag test P.f/Pan® kit with the current gold standard diagnosis by microscopy. The prevalence of pfhrp2 and pfhrp3 deletions were evaluated from 221 P. falciparum isolates collected between 2009 and 2011 in French Guiana.
resultsBetween January 2010 and August 2011, 960 suspected cases of malaria were analyzed using microscopy and RDTs. The sensitivity of the SD Malaria Ag test P.f/Pan® for detection of P. falciparum was 96.8% (95% CI: 90.9-99.3), and 86.0% (95% CI: 78.9-91.5) for the detection of P. vivax. No isolates (95% CI: 0-4.5) lacking either exon of the pfhrp2 gene were identified among the 221 P. falciparum isolates analyzed, but 7.4% (95% CI: 2.8-15.4) lacked the exon 2 part of the pfhrp3 gene.
conclusionsField isolates lacking either exon of the pfhrp2 gene are absent in this western part of South America. Despite its sensibility to detect P. vivax, the SD Malaria Ag test P.f/Pan® kit is a satisfying alternative to microscopy in remote health centers, where it is difficult to provide highly skilled microscopists and to maintain the necessary equipment.
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