ArticlePloS one2013
Gene regulatory networks elucidating huanglongbing disease mechanisms.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 3 of them syntheses that pooled it.
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Who cites it
49 citing papers in PubMed, 3 syntheses or guidelines pooled it, 116 citations in OpenAlex.
- Identification of key genes and its chromosome regions linked to drought responses in leaves across different crops through meta-analysis of RNA-Seq data.BMC plant biology · 2019Pooled it
- Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.Scientific reports · 2018Pooled it
- Comprehensive meta-analysis, co-expression, and miRNA nested network analysis identifies gene candidates in citrus against Huanglongbing disease.BMC plant biology · 2015Pooled it
- Exploring the biochemical and molecular mechanisms that contribute to Huanglongbing (HLB) tolerance in Citrus australis hybrids.BMC genomics · 2025Article
- Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues.Plants (Basel, Switzerland) · 2025Article
- A Metabolomics Assay to Diagnose Citrus Huanglongbing Disease and to Aid in Assessment of Treatments to Prevent or Cure Infection.Phytopathology · 2024Article
- Overexpressing CsSABP2 enhances tolerance to Huanglongbing and citrus canker inFrontiers in plant science · 2024Article
- Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes withinFrontiers in plant science · 2024Article
- Dust Under the Radar: Rethinking How to Evaluate the Impacts of Dust Events on Air Quality in the United States.GeoHealth · 2023Article
- Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of "Candidatus Liberibacter asiaticus" in citrus.Molecular horticulture · 2023Article
- Unraveling the mystery of canopy dieback caused by citrus disease Huanglongbing and its link to hypoxia stress.Frontiers in plant science · 2023Article
- How Stress Affects Your Budget-Stress Impacts on Starch Metabolism.Frontiers in plant science · 2022Review
- Molecular signatures between citrus and Candidatus Liberibacter asiaticus.PLoS pathogens · 2021Review
- Overexpression of Salicylic Acid Carboxyl Methyltransferase (International journal of molecular sciences · 2021Article
- Transcriptomic Response of Huanglongbing-InfectedFrontiers in plant science · 2021Article
- Overexpression of a "Frontiers in microbiology · 2021Article
- Comparative Proteomic Analysis of Sweet Orange Petiole Provides Insights Into the Development of Huanglongbing Symptoms.Frontiers in plant science · 2021Article
- Revisiting the Complex Pathosystem of Huanglongbing: Deciphering the Role of Citrus Metabolites in Symptom Development.Metabolites · 2020Review
- Longitudinal Transcriptomic, Proteomic, and Metabolomic Analysis ofJournal of proteome research · 2020Article
- Heterologous Expression of CLIBASIA_03915/CLIBASIA_04250 byInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Next-generation sequencing was exploited to gain deeper insight into the response to infection by Candidatus liberibacter asiaticus (CaLas), especially the immune disregulation and metabolic dysfunction caused by source-sink disruption. Previous fruit transcriptome data were compared with additional RNA-Seq data in three tissues: immature fruit, and young and mature leaves. Four categories of orchard trees were studied: symptomatic, asymptomatic, apparently healthy, and healthy. Principal component analysis found distinct expression patterns between immature and mature fruits and leaf samples for all four categories of trees. A predicted protein - protein interaction network identified HLB-regulated genes for sugar transporters playing key roles in the overall plant responses. Gene set and pathway enrichment analyses highlight the role of sucrose and starch metabolism in disease symptom development in all tissues. HLB-regulated genes (glucose-phosphate-transporter, invertase, starch-related genes) would likely determine the source-sink relationship disruption. In infected leaves, transcriptomic changes were observed for light reactions genes (downregulation), sucrose metabolism (upregulation), and starch biosynthesis (upregulation). In parallel, symptomatic fruits over-expressed genes involved in photosynthesis, sucrose and raffinose metabolism, and downregulated starch biosynthesis. We visualized gene networks between tissues inducing a source-sink shift. CaLas alters the hormone crosstalk, resulting in weak and ineffective tissue-specific plant immune responses necessary for bacterial clearance. Accordingly, expression of WRKYs (including WRKY70) was higher in fruits than in leaves. Systemic acquired responses were inadequately activated in young leaves, generally considered the sites where most new infections occur.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.