ArticlePloS one2013
Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- The fate of artificial transgenes in Acanthamoeba castellanii.BMC genomics · 2025Article
- Characterization of Glucokinases from Pathogenic Free-Living Amoebae.Antimicrobial agents and chemotherapy · 2022Article
- Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics.BMC biology · 2022Article
- Incomplete tricarboxylic acid cycle and proton gradient in Pandoravirus massiliensis: is it still a virus?The ISME journal · 2022Article
- Pangenomics reveals alternative environmental lifestyles among chlamydiae.Nature communications · 2021Article
- Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation.Biology · 2020Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Anaerobic peroxisomes inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Energy metabolism in anaerobic eukaryotes and Earth's late oxygenation.Free radical biology & medicine · 2019Review
- Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii.Parasites & vectors · 2018Article
- The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup.BMC genomics · 2017Article
- Anaerobic Metabolism in T4 Acanthamoeba Genotype.Current microbiology · 2017Article
- Novel Hydrogenosomes in the Microaerophilic Jakobid Stygiella incarcerata.Molecular biology and evolution · 2016Article
- Animals, anoxic environments, and reasons to go deep.BMC biology · 2016Article
- Diversity and origins of anaerobic metabolism in mitochondria and related organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Data showing the compositional complexity of the mitochondrial proteome of a unicellular eukaryote (Acanthamoeba castellanii, supergroup Amoebozoa).Data in brief · 2014Article
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4 authors at 1 institution in 1 country.
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Abstract
Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes.
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