ArticlePLoS genetics2013
Binding of NF-κB to nucleosomes: effect of translational positioning, nucleosome remodeling and linker histone H1.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Transposon invasion of primate genomes shaped human inflammatory enhancers and susceptibility to inflammatory diseases.Nature communications · 2025Article
- Time-varying stimuli that prolong IKK activation promote nuclear remodeling and mechanistic switching of NF-κB dynamics.Nature communications · 2025Article
- The transcription factor NF-κB orchestrates nucleosome remodeling during the primary response to Toll-like receptor 4 signaling.Immunity · 2024Article
- Development of a mouse embryonic stem cell model for investigating the functions of the linker histone H1-4.FEBS open bio · 2024Article
- Molecular Mechanism of Nucleosome Recognition by the Pioneer Transcription Factor Sox.Journal of chemical information and modeling · 2023Article
- Functional Hallmarks of Healthy Macrophage Responses: Their Regulatory Basis and Disease Relevance.Annual review of immunology · 2022Review
- Epigenetic memory contributing to the pathogenesis of AKI-to-CKD transition.Frontiers in molecular biosciences · 2022Review
- Epigenetic Regulation of Inflammatory Signaling and Inflammation-Induced Cancer.Frontiers in cell and developmental biology · 2022Review
- Effect of histone H4 tail on nucleosome stability and internucleosomal interactions.Scientific reports · 2021Article
- NF-κB Rel subunit exchange on a physiological timescale.Protein science : a publication of the Protein Society · 2021Article
- Transcription factor NF-κB unravels nucleosomes.Biochimica et biophysica acta. General subjects · 2021Article
- NF-κB dynamics determine the stimulus specificity of epigenomic reprogramming in macrophages.Science (New York, N.Y.) · 2021Article
- TheCancer research · 2021Article
- Signaling-to-chromatin pathways in the immune system.Immunological reviews · 2021Review
- The histone demethylase KDM6B fine-tunes the host response to Streptococcus pneumoniae.Nature microbiology · 2021Article
- Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals.Nature communications · 2021Article
- Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry.Nature communications · 2020Article
- MNase Profiling of Promoter Chromatin inG3 (Bethesda, Md.) · 2020Article
- Pioneer factors and their in vitro identification methods.Molecular genetics and genomics : MGG · 2020Review
- Genome reading by the NF-κB transcription factors.Nucleic acids research · 2019Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser footprinting, hydroxyl footprinting and electrophoretic mobility shift assay to investigate the binding of NF-κB to nucleosomal templates. We show that NF-κB p50 homodimer is able to bind to its recognition sequence, when it is localized at the edge of the core particle, but not when the recognition sequence is at the interior of the nucleosome. Remodeling of the nucleosome by the chromatin remodeling machine RSC was not sufficient to allow binding of NF-κB to its recognition sequence located in the vicinity of the nucleosome dyad, but RSC-induced histone octamer sliding allowed clearly detectable binding of NF-κB with the slid particle. Importantly, nucleosome dilution-driven removal of H2A-H2B dimer led to complete accessibility of the site located close to the dyad to NF-κB. Finally, we found that NF-κB was able to displace histone H1 and prevent its binding to nucleosome. These data provide important insight on the role of chromatin structure in the regulation of transcription of NF-κB dependent genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.