ArticleJournal of visualized experiments : JoVE2013
Culturing and maintaining Clostridium difficile in an anaerobic environment.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 98 citations in OpenAlex.
- Multi-strain Clostridioides difficile infection: Increased detection and clinical implications.The Journal of infection · 2026Article
- Optimized Surveillance Testing for ToxigenicmedRxiv : the preprint server for health sciences · 2026Article
- Phase variable colony morphotypes ofInfection and immunity · 2026Article
- Contact dependent suppression ofbioRxiv : the preprint server for biology · 2026Article
- A streamlined, nanopore-compatible 5PSeq protocol for rapid phenotypic antimicrobial sensitivity testing.Cell reports methods · 2026Article
- Proteomic antibacterial characterization of flavonoid xanthohumol and probiotic Clostridium butyricum on pathogenic Clostridioides difficile.Chinese medicine · 2026Article
- Phase variable colony morphotypes ofbioRxiv : the preprint server for biology · 2026Article
- Bridging the gap in AMR research: validation of a standardizedFrontiers in pharmacology · 2026Article
- Dynamic microbiota in water kefir: microbial shift and ecological selection during fermentation.BMC microbiology · 2025Article
- A Colombian strain ofVirulence · 2025Article
- A targeted cell lysis mechanism facilitates toxin release in Clostridioides difficile.bioRxiv : the preprint server for biology · 2025Article
- The Pxp Complex Detoxifies 5-Oxoproline and Promotes the Growth of Clostridioides difficile.Molecular microbiology · 2025Article
- A metabolite dehydrogenase pathway represses sporulation of Clostridioides difficile.Anaerobe · 2025Article
- Potent Antimicrobial Activity ofAntibiotics (Basel, Switzerland) · 2025Article
- Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives.Computational and structural biotechnology journal · 2025Review
- Comparative analysis of the postadmission and antemortem oropharyngeal and rectal swab microbiota of ICU patients.Scientific reports · 2024Article
- Unraveling the Gut Microbiota: Implications for Precision Nutrition and Personalized Medicine.Nutrients · 2024Review
- Role of glycogen metabolism inmSphere · 2024Article
- Purified CDT toxins and a clean deletion within the CDT locus provide novel insights into the contribution of binary toxin in cellular inflammation and Clostridioides difficile infection.PLoS pathogens · 2024Article
- Review
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Clostridium difficile is a Gram-positive, anaerobic, sporogenic bacterium that is primarily responsible for antibiotic associated diarrhea (AAD) and is a significant nosocomial pathogen. C. difficile is notoriously difficult to isolate and cultivate and is extremely sensitive to even low levels of oxygen in the environment. Here, methods for isolating C. difficile from fecal samples and subsequently culturing C. difficile for preparation of glycerol stocks for long-term storage are presented. Techniques for preparing and enumerating spore stocks in the laboratory for a variety of downstream applications including microscopy and animal studies are also described. These techniques necessitate an anaerobic chamber, which maintains a consistent anaerobic environment to ensure proper conditions for optimal C. difficile growth. We provide protocols for transferring materials in and out of the chamber without causing significant oxygen contamination along with suggestions for regular maintenance required to sustain the appropriate anaerobic environment for efficient and consistent C. difficile cultivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.