ArticleJournal of visualized experiments : JoVE2013
A method for culturing embryonic C. elegans cells.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- SVHRSP Attenuates PTZ-Induced Seizure-Like Behavior in C. elegans: Involvement of HSF-1 and Associated Changes in GABAergic Markers and Mitochondrial Homeostasis.Neurochemical research · 2026Article
- Comparative analysis of nanosilver toxicity in C. elegans: influence of exposure media on accumulation, physiological and biochemical effects.Environmental science and pollution research international · 2026Article
- C. elegans touch receptor neurons direct mechanosensory complex organization via repurposing conserved basal lamina proteins.Current biology : CB · 2024Article
- Article
- Phenotypic Screening inPharmaceuticals (Basel, Switzerland) · 2020Review
- Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms.Scientific reports · 2019Article
- Knock-out of a mitochondrial sirtuin protects neurons from degeneration in Caenorhabditis elegans.PLoS genetics · 2017Article
- A Novel Mechanism of pH Buffering in C. elegans Glia: Bicarbonate Transport via the Voltage-Gated ClC Cl- Channel CLH-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Mechanosensitive unpaired innexin channels in C. elegans touch neurons.American journal of physiology. Cell physiology · 2014Article
- NRA-2, a nicalin homolog, regulates neuronal death by controlling surface localization of toxic Caenorhabditis elegans DEG/ENaC channels.The Journal of biological chemistry · 2014Article
- Caenorhabditis elegans glia modulate neuronal activity and behavior.Frontiers in cellular neuroscience · 2014Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C. elegans is a powerful model system, in which genetic and molecular techniques are easily applicable. Until recently though, techniques that require direct access to cells and isolation of specific cell types, could not be applied in C. elegans. This limitation was due to the fact that tissues are confined within a pressurized cuticle which is not easily digested by treatment with enzymes and/or detergents. Based on early pioneer work by Laird Bloom, Christensen and colleagues developed a robust method for culturing C. elegans embryonic cells in large scale. Eggs are isolated from gravid adults by treatment with bleach/NaOH and subsequently treated with chitinase to remove the eggshells. Embryonic cells are then dissociated by manual pipetting and plated onto substrate-covered glass in serum-enriched media. Within 24 hr of isolation cells begin to differentiate by changing morphology and by expressing cell specific markers. C. elegans cells cultured using this method survive for up 2 weeks in vitro and have been used for electrophysiological, immunochemical, and imaging analyses as well as they have been sorted and used for microarray profiling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.