ArticleJournal of visualized experiments : JoVE2013
The Tomato/GFP-FLP/FRT method for live imaging of mosaic adult Drosophila photoreceptor cells.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.Acta neuropathologica communications · 2022Article
- Article
- Fly-QMA: Automated analysis of mosaic imaginal discs in Drosophila.PLoS computational biology · 2020Article
- Drosophila p53 integrates the antagonism between autophagy and apoptosis in response to stress.Autophagy · 2019Article
- TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals.PLoS genetics · 2019Article
- Physiological and pathological roles of FATP-mediated lipid droplets in Drosophila and mice retina.PLoS genetics · 2018Article
- Article
- Methods for studying planar cell polarity.Methods (San Diego, Calif.) · 2014Review
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Authors and funding
5 authors.
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Abstract
The Drosophila eye is widely used as a model for studies of development and neuronal degeneration. With the powerful mitotic recombination technique, elegant genetic screens based on clonal analysis have led to the identification of signaling pathways involved in eye development and photoreceptor (PR) differentiation at larval stages. We describe here the Tomato/GFP-FLP/FRT method, which can be used for rapid clonal analysis in the eye of living adult Drosophila. Fluorescent photoreceptor cells are imaged with the cornea neutralization technique, on retinas with mosaic clones generated by flipase-mediated recombination. This method has several major advantages over classical histological sectioning of the retina: it can be used for high-throughput screening and has proved an effective method for identifying the factors regulating PR survival and function. It can be used for kinetic analyses of PR degeneration in the same living animal over several weeks, to demonstrate the requirement for specific genes for PR survival or function in the adult fly. This method is also useful for addressing cell autonomy issues in developmental mutants, such as those in which the establishment of planar cell polarity is affected.
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