ArticleAnalytical chemistry2013
Microfluidic array with integrated oxygenation control for real-time live-cell imaging: effect of hypoxia on physiology of microencapsulated pancreatic islets.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Engineering the oxygen microenvironment for pancreatic islet transplants.NPJ Regenerative medicine · 2026Review
- Microfluidics, 3D bioprinted scaffold and organ-on-chip with biosensing activated therapeutic strategies for diabetic wound healing.Frontiers in bioengineering and biotechnology · 2026Review
- The future of islet transplantation beyond the BLA approval: challenges and opportunities.Frontiers in transplantation · 2025Review
- Visualizing hypoxic modulation of beta cell secretions via a sensor augmented oxygen gradient.Microsystems & nanoengineering · 2023Article
- Pancreatic islet organoids-on-a-chip: how far have we gone?Journal of nanobiotechnology · 2022Review
- Smartphone-microfluidic fluorescence imaging system for studying islet physiology.Frontiers in endocrinology · 2022Article
- A therapeutic vascular conduit to support in vivo cell-secreted therapy.NPJ Regenerative medicine · 2021Article
- 96-Well Oxygen Control Using a 3D-Printed Device.Analytical chemistry · 2021Article
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- A Parallel Perifusion Slide From Glass for the Functional and Morphological Analysis of Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- Pancreas-on-a-Chip Technology for Transplantation Applications.Current diabetes reports · 2020Review
- In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution.Advanced biosystems · 2020Article
- Microfluidics for interrogating live intact tissues.Microsystems & nanoengineering · 2020Review
- Synchronized stimulation and continuous insulin sensing in a microfluidic human Islet on a Chip designed for scalable manufacturing.Lab on a chip · 2019Article
- Microfluidic Cell Trapping for Single-Cell Analysis.Micromachines · 2019Article
- Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations.The Analyst · 2019Review
- Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques.Nature biomedical engineering · 2018Article
- 3D-Models of Insulin-Producing β-Cells: from Primary Islet Cells to Stem Cell-Derived Islets.Stem cell reviews and reports · 2018Review
- Hypoxia-enhanced adhesion of red blood cells in microscale flow.Microcirculation (New York, N.Y. : 1994) · 2017Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
In this article, we present a novel microfluidic islet array based on a hydrodynamic trapping principle. The lab-on-a-chip studies with live-cell multiparametric imaging allow understanding of physiological and pathophysiological changes of microencapsulated islets under hypoxic conditions. Using this microfluidic array and imaging analysis techniques, we demonstrate that hypoxia impairs the function of microencapsulated islets at the single islet level, showing a heterogeneous pattern reflected in intracellular calcium signaling, mitochondrial energetic, and redox activity. Our approach demonstrates an improvement over conventional hypoxia chambers that is able to rapidly equilibrate to true hypoxia levels through the integration of dynamic oxygenation. This work demonstrates the feasibility of array-based cellular analysis and opens up new modality to conduct informative analysis and cell-based screening for microencapsulated pancreatic islets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.