ArticleMicrobial cell factories2013
Identification of factors for improved ethylene production via the ethylene forming enzyme in chemostat cultures of Saccharomyces cerevisiae.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Purification-free antifungal biocontrol platform using engineered Saccharomyces cerevisiae secreting Trichoderma atroviride chitinase.Bioresources and bioprocessing · 2026Article
- Uncoupling protein production from growth: different strategies for intracellular and secreted proteins in yeast.Microbial cell factories · 2025Article
- Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803.NPJ systems biology and applications · 2023Article
- Metabolic engineering of Zymomonas moblis for ethylene production from straw hydrolysate.Applied microbiology and biotechnology · 2021Article
- Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization.Microbial cell factories · 2018Article
- Bio-production of gaseous alkenes: ethylene, isoprene, isobutene.Biotechnology for biofuels · 2018Review
- Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.Journal of the American Chemical Society · 2017Article
- Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.Biochemistry · 2016Article
- Maintenance-energy requirements and robustness of Saccharomyces cerevisiae at aerobic near-zero specific growth rates.Microbial cell factories · 2016Article
- Growth-rate dependency of de novo resveratrol production in chemostat cultures of an engineered Saccharomyces cerevisiae strain.Microbial cell factories · 2015Article
- Engineering biological systems toward a sustainable bioeconomy.Journal of industrial microbiology & biotechnology · 2015Review
- Comparative sequence analysis and mutagenesis of ethylene forming enzyme (EFE) 2-oxoglutarate/Fe(II)-dependent dioxygenase homologs.BMC biochemistry · 2014Article
- Ethylene-forming enzyme and bioethylene production.Biotechnology for biofuels · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiotechnological production of the traditional petrochemical ethylene is presently being explored using yeasts as well as bacteria. In this study we quantify the specific ethylene production levels at different conditions in continuous (chemostat) cultivation of Saccharomyces cerevisae expressing the ethylene forming enzyme (EFE) from Pseudomonas syringae.
resultsOur study shows that oxygen availability is an important factor for the ethylene formation. Maintaining a high percentage dissolved oxygen in the cultivation was found to be necessary to achieve maximal ethylene productivity. Even at oxygen levels high enough to sustain respiratory metabolism the ethylene formation was restricted. Oxygen was also important for sustaining a high respiratory rate and to re-oxidize the surplus of NADH that accompanies ethylene formation. By employing three different nitrogen sources we further found that the nitrogen source available can both improve and impair the ethylene productivity. Contrary to findings in batch cultures, using glutamate did not give a significant increase in specific ethylene production levels compared to the reference condition with ammonia, whereas a combination of glutamate and arginine resulted in a strongly diminished specific ethylene production. Furthermore, from cultivations at different dilution rates the ethylene formation was found to be coupled to growth rate.
conclusionTo optimize the ethylene productivity in S. cerevisiae expressing a bacterial ethylene forming enzyme, controlling the oxygen availability and growth rate as well as employing an ideal nitrogen source is of importance. The effects of these factors as studied here provide a basis for an optimized process for ethylene production in S. cerevisiae.
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