ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2012
Proteolytic activities in Phaseolus vulgaris cotyledons under copper stress.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Does Folcisteine (NATCA) Play a Role in Facilitating Seed Germination, Root Development, and Elevating Root AsA-GSH Cycle Efficiency Under Combined Copper-Cadmium Stress in Maize?International journal of molecular sciences · 2025Article
- Article
- Production of Dipeptidyl Peptidase-IV Inhibitory Peptides from Germinated Jack Bean [Preventive nutrition and food science · 2023Article
- Alleviation of Copper-Induced Stress in Pea (Biology · 2021Article
- Heavy metal-induced oxidative stress on seed germination and seedling development: a critical review.Environmental geochemistry and health · 2019Review
- Article
- Effects of copper on reserve mobilization in embryo of Phaseolus vulgaris L.Environmental science and pollution research international · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
The changes in the protease activities of bean cotyledons were investigated in response to copper stress. Assays using synthetic substrates and specific protease inhibitors followed by activity measurements and electrophoresis analysis allowed to study the classes of enzymes involved in the storage protein mobilization during the germination of bean (Phaseolus vulgaris L) seeds, and then identify which ones were affected in the presence of 200 μM CuCl2 in the imbibition medium. Copper treatment affected embryo growth and total protease activity. The results of SDS-gelatin-PAGE show that Cu excess led to a decrease in protease activity of 45 to 66 kDa. Moreover, cysteine-, aspartic- and metallo-protease activities were markedly lowered under copper stress, while serine-protease one was enhanced as well as its activity dependent abundance in comparison with control. However, the relative distribution of major cysteine protease in H2O-germinated seeds was significantly diminished after Cu exposure. Thus, copper excess can disturb the nitrogen freeing from reserve tissues at enzymatic level; differential responses of protease classes are discussed, notably, cysteine protease in the way of storage protein mobilization and serine protease in protective mechanism one.
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