ArticleProceedings of the National Academy of Sciences of the United States of America2013
In vitro evolution of α-hemolysin using a liposome display.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 143 citations in OpenAlex.
- Combinatorial Optimization of Protein Systems in Synthetic Cells.ACS synthetic biology · 2026Article
- Image-based phenotypic sorting of synthetic cells.Science advances · 2026Article
- Engineering Liposomes and Polymer Conjugates: A Platform for Mechanistic Complement Activation Studies and Controlled Release Applications.ACS applied materials & interfaces · 2026Article
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- Functional reconstitution of bacterial ESCRT-III protein PspA identifies key regions in membrane binding and remodeling.Protein science : a publication of the Protein Society · 2025Article
- Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell.Nature communications · 2024Article
- Applications of cell free protein synthesis in protein design.Protein science : a publication of the Protein Society · 2024Review
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- Progresses in Cell-Free In Vitro Evolution.Advances in biochemical engineering/biotechnology · 2023Review
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Synthetic Cells: From Simple Bio-Inspired Modules to Sophisticated Integrated Systems.Angewandte Chemie (International ed. in English) · 2022Review
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- Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules.ACS synthetic biology · 2021Article
- Article
- System concentration shift as a regulator of transcription-translation system within liposomes.iScience · 2021Article
- More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display.RSC advances · 2020Article
- Genetically controlled membrane synthesis in liposomes.Nature communications · 2020Article
- Hydrodynamic accumulation of small molecules and ions into cell-sized liposomes against a concentration gradient.Communications chemistry · 2020Article
- Protocells programmed through artificial reaction networks.Chemical science · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro methods have enabled the rapid and efficient evolution of proteins and successful generation of novel and highly functional proteins. However, the available methods consider only globular proteins (e.g., antibodies, enzymes) and not membrane proteins despite the biological and pharmaceutical importance of the latter. In this study, we report the development of a method called liposome display that can evolve the properties of membrane proteins entirely in vitro. This method, which involves in vitro protein synthesis inside liposomes, which are cell-sized phospholipid vesicles, was applied to the pore-forming activity of α-hemolysin, a membrane protein derived from Staphylococcus aureus. The obtained α-hemolysin mutant possessed only two point mutations but exhibited a 30-fold increase in its pore-forming activity compared with the WT. Given the ability to synthesize various membrane proteins and modify protein synthesis and functional screening conditions, this method will allow for the rapid and efficient evolution of a wide range of membrane proteins.
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