ArticleProceedings of the National Academy of Sciences of the United States of America2013
Discovery of novel chemoeffectors and rational design of Escherichia coli chemoreceptor specificity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Chemotaxis to plant defense compounds in phytopathogens.PLoS pathogens · 2026Article
- Navigated range expansion promotes migratory culling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of a dCache-type chemoreceptor inFrontiers in microbiology · 2024Article
- Attractant and repellent induce opposing changes in the four-helix bundle ligand-binding domain of a bacterial chemoreceptor.PLoS biology · 2023Article
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- Coevolution-based prediction of key allosteric residues for protein function regulation.eLife · 2023Article
- Real-time detection of response regulator phosphorylation dynamics in live bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Discovery of a New Chemoeffector forACS bio & med chem Au · 2022Article
- Hexameric rings of the scaffolding protein CheW enhance response sensitivity and cooperativity inScience signaling · 2022Article
- N-Formyl-L-aspartate mediates chemotaxis in spermFrontiers in cell and developmental biology · 2022Article
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- Production and Characterization of Motile and Chemotactic Bacterial Minicells.ACS synthetic biology · 2021Article
- Review
- Hybrid Two-Component Sensors for Identification of Bacterial Chemoreceptor Function.Applied and environmental microbiology · 2019Article
- Article
- The activity of the C4-dicarboxylic acid chemoreceptor of Pseudomonas aeruginosa is controlled by chemoattractants and antagonists.Scientific reports · 2018Article
- Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient.Scientific reports · 2017Article
- Logistic Regression of Ligands of Chemotaxis Receptors Offers Clues about Their Recognition by Bacteria.Frontiers in bioengineering and biotechnology · 2017Article
- Structural basis for ligand recognition by a Cache chemosensory domain that mediates carboxylate sensing in Pseudomonas syringae.Scientific reports · 2016Article
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Authors and funding
10 authors at 7 institutions in 4 countries.
Funding
Abstract
Bacterial chemoreceptors mediate chemotactic responses to diverse stimuli. Here, by using an integrated in silico, in vitro, and in vivo approach, we screened a large compound library and found eight novel chemoeffectors for the Escherichia coli chemoreceptor Tar. Six of the eight new Tar binding compounds induce attractant responses, and two of them function as antagonists that can bind Tar without inducing downstream signaling. Comparison between the antagonist and attractant binding patterns suggests that the key interactions for chemotaxis signaling are mediated by the hydrogen bonds formed between a donor group in the attractant and the main-chain carbonyls (Y149 and/or Q152) on the α4 helix of Tar. This molecular insight for signaling is verified by converting an antagonist to an attractant when introducing an N-H group into the antagonist to restore the hydrogen bond. Similar signal triggering effect by an O-H group is also confirmed. Our study suggests that the Tar chemoeffector binding pocket may be separated into two functional regions: region I mainly contributes to binding and region II contributes to both binding and signaling. This scenario of binding and signaling suggests that Tar may be rationally designed to respond to a nonnative ligand by altering key residues in region I to strengthen binding with the novel ligand while maintaining the key interactions in region II for signaling. Following this strategy, we have successfully redesigned Tar to respond to l-arginine, a basic amino acid that does not have chemotactic effect for WT Tar, by two site-specific mutations (R69'E and R73'E).
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