ArticlePlant physiology2013
Analyses of Ca2+ accumulation and dynamics in the endoplasmic reticulum of Arabidopsis root cells using a genetically encoded Cameleon sensor.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Cell-type-specific immune programs orchestrate spatial defense in the Arabidopsis leaf epidermis.Nature communications · 2026Article
- The photosensitive endoplasmic reticulum-chloroplast contact site.Journal of microscopy · 2025Article
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.The EMBO journal · 2023Article
- Calcium Live Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2023Article
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- Endoplasmic reticulum oxidoreductin provides resilience against reductive stress and hypoxic conditions by mediating luminal redox dynamics.The Plant cell · 2022Article
- Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots.Journal of experimental botany · 2022Article
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments.Plant physiology · 2022Article
- Monitoring calcium handling by the plant endoplasmic reticulum with a low-CaThe Plant journal : for cell and molecular biology · 2022Article
- Biological and Molecular Components for Genetically Engineering Biosensors in Plants.Biodesign research · 2022Article
- The signatures of organellar calcium.Plant physiology · 2021Review
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Illuminating the hidden world of calcium ions in plants with a universe of indicators.Plant physiology · 2021Review
- Simultaneous imaging of ER and cytosolic Ca2+ dynamics reveals long-distance ER Ca2+ waves in plants.Plant physiology · 2021Article
- Phylogenetic and ion-response analyses reveal a relationship between gene expansion and functional divergence in the CaPlant molecular biology · 2021Article
- The plant endoplasmic reticulum: an organized chaos of tubules and sheets with multiple functions.Journal of microscopy · 2020Review
- Stay in Touch-The Cortical ER of Moss Protonemata in Osmotic Stress Situations.Plants (Basel, Switzerland) · 2020Article
- DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP.Annals of botany · 2020Article
- Phosphate Starvation Alters Abiotic-Stress-Induced Cytosolic Free Calcium Increases in Roots.Plant physiology · 2019Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In planta, very limited information is available about how the endoplasmic reticulum (ER) contributes to cellular Ca(2+) dynamics and homeostasis. Here, we report the generation of an ER-targeted Cameleon reporter protein suitable for analysis of Ca(2+) accumulation and dynamics in the lumen of the ER in plant cells. Using stably transformed Arabidopsis (Arabidopsis thaliana) plants expressing this reporter protein, we observed a transiently enhanced accumulation of Ca(2+) in the ER in response to stimuli inducing cytosolic Ca(2+) rises in root tip cells. In all experimental conditions, ER Ca(2+) dynamics were substantially different from those monitored in the cytosol. A pharmacological approach enabled us to evaluate the contribution of the different ER-resident Ca(2+)-ATPase classes in the regulation of the ER Ca(2+) homeostasis. Taken together, our results do not provide evidence for a role of the ER as a major source that releases Ca(2+) for stimulus-induced increases in cytosolic Ca(2+) concentration. Instead, our results show that the luminal ER Ca(2+) elevations typically follow cytosolic ones, but with distinct dynamics. These findings suggest fundamental differences for the function of the ER in cellular Ca(2+) homeostasis in plants and animals.
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