ArticleCurrent genetics2014
Activities and specificities of homodimeric TALENs in Saccharomyces cerevisiae.
Article in Current genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Methods of crop improvement and applications towards fortifying food security.Frontiers in genome editing · 2023Review
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Targeted introduction of heritable point mutations into the plant mitochondrial genome.Nature plants · 2022Article
- High-efficiency retron-mediated single-stranded DNA production in plants.Synthetic biology (Oxford, England) · 2022Article
- Synthetic directed evolution in plants: unlocking trait engineering and improvement.Synthetic biology (Oxford, England) · 2021Review
- Autoimmunity in plants.Planta · 2018Review
- Article
- CAR1 deletion by CRISPR/Cas9 reduces formation of ethyl carbamate from ethanol fermentation by Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2016Article
- Genome editing: the road of CRISPR/Cas9 from bench to clinic.Experimental & molecular medicine · 2016Review
- High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease.Plant cell reports · 2016Article
- Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi.The plant pathology journal · 2016Article
- CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion.Scientific reports · 2016Article
- Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance.Frontiers in plant science · 2016Review
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering.PloS one · 2015Article
- Activity and specificity of TRV-mediated gene editing in plants.Plant signaling & behavior · 2015Article
- Complete decoding of TAL effectors for DNA recognition.Cell research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of highly efficient genome engineering reagents is of paramount importance to launch the next wave of biotechnology. TAL effectors have been developed as an adaptable DNA binding scaffold that can be engineered to bind to any user-defined sequence. Thus, TAL-based DNA binding modules have been used to generate chimeric proteins for a variety of targeted genome modifications across eukaryotic species. For example, TAL effectors fused to the catalytic domain of FokI endonuclease (TALENs) were used to generate site-specific double strand breaks (DSBs), the repair of which can be harnessed to dictate user-desired, genome-editing outcomes. To cleave DNA, FokI endonuclease must dimerize which can be achieved using a pair of TALENs that bind to the DNA targeted in a tail-to-tail orientation with proper spacing allowing the dimer formation. Because TALENs binding to DNA are dependent on their repeat sequences and nucleotides binding specificities, homodimers and heterodimers binding can be formed. In the present study, we used several TALEN monomers with increased repeats binding degeneracy to allow homodimer formation at increased number of genomic loci. We assessed their binding specificities and genome modification activities. Our results indicate that homodimeric TALENs could be used to modify the yeast genome in a site-specific manner and their binding to the promoter regions might modulate the expression of target genes. Taken together, our data indicate that homodimeric TALENs could be used to achieve different engineering possibilities of biotechnological applications and that their transcriptional modulations need to be considered when analyzing their phenotypic effects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.