ArticleThe Journal of physiology2013
Myosin filaments in smooth muscle cells do not have a constant length.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Smooth muscle contractile cytoskeleton in health and disease.Journal of muscle research and cell motility · 2026Review
- Chemo-mechanical modeling of smooth muscle cell activation for the simulation of arterial walls under changing blood pressure.Biomechanics and modeling in mechanobiology · 2023Article
- Self-assembly of smooth muscle myosin filaments: adaptation of filament length by telokin and Mg·ATP.European biophysics journal : EBJ · 2022Article
- Actomyosin Complex.Sub-cellular biochemistry · 2022Article
- Filament evanescence of myosin II and smooth muscle function.The Journal of general physiology · 2021Review
- Evidence for S2 flexibility by direct visualization of quantum dot-labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro.The Journal of general physiology · 2021Article
- The Force Awakens in the Cytoskeleton: The Saga of a Shape-Shifter.American journal of respiratory cell and molecular biology · 2020Article
- Fundamental Roles of Axial Stretch in Isometric and Isobaric Evaluations of Vascular Contractility.Journal of biomechanical engineering · 2019Article
- Myosin Crossbridge, Contractile Unit, and the Mechanism of Contraction in Airway Smooth Muscle: A Mechanical Engineer's Perspective.Journal of engineering and science in medical diagnostics and therapy · 2019Review
- Ordering of myosin II filaments driven by mechanical forces: experiments and theory.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Multiscale and Multiaxial Mechanics of Vascular Smooth Muscle.Biophysical journal · 2017Article
- Adaptation of active tone in the mouse descending thoracic aorta under acute changes in loading.Biomechanics and modeling in mechanobiology · 2016Article
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Phosphate and ADP differently inhibit coordinated smooth muscle myosin groups.Biophysical journal · 2015Article
- Reply from Chun Y. Seow.The Journal of physiology · 2015Article
- Smooth muscle myosin filament controversy, once again?The Journal of physiology · 2015Article
- Force maintenance and myosin filament assembly regulated by Rho-kinase in airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Emergence of airway smooth muscle mechanical behavior through dynamic reorganization of contractile units and force transmission pathways.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
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10 authors at 1 institution in 1 country.
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Abstract
Myosin molecules from smooth muscle and non-muscle cells are known to self-assemble into side-polar filaments in vitro. However, the in situ mechanism of filament assembly is not clear and the question of whether there is a unique length for myosin filaments in smooth muscle is still under debate. In this study we measured the lengths of 16,587 myosin filaments in three types of smooth muscle cells using serial electron microscopy (EM). Sheep airway and pulmonary arterial smooth muscle as well as rabbit carotid arterial smooth muscle were fixed for EM and serial ultra-thin (50-60 nm) sections were obtained. Myosin filaments were traced in consecutive sections to determine their lengths. The results indicate that there is not a single length for the myosin filaments; instead there is a wide variation in lengths. The plots of observation frequency versus myosin filament length follow an exponential decay pattern. Analysis suggests that in situ assembly of myosin filaments in smooth muscle is governed by random processes of linear polymerization and de-polymerization, and that the dynamic equilibrium of these processes determines the observed length distribution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.