ArticleOptics letters2013
Active wide-field illumination for high-throughput fluorescence lifetime imaging.
Article in Optics letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Medium-adaptive compressive diffuse optical tomography.Biomedical optics express · 2024Article
- Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.Theranostics · 2020Article
- Fast fit-free analysis of fluorescence lifetime imaging via deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short-lifetime near-infrared dyes and time-gated imaging.Journal of biophotonics · 2019Article
- Comparison of illumination geometry for lifetime-based measurements in whole-body preclinical imaging.Journal of biophotonics · 2018Article
- Quantitative imaging of receptor-ligand engagement in intact live animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Review of structured light in diffuse optical imaging.Journal of biomedical optics · 2018Review
- AlliGator: A Phasor Computational Platform for FastOptical molecular probes, imaging and drug delivery · 2017Article
- Assessment of Gate Width Size on Lifetime-Based Förster Resonance Energy Transfer Parameter Estimation.Photonics · 2015Article
- Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging.PloS one · 2015Article
- Reduced temporal sampling effect on accuracy of time-domain fluorescence lifetime Förster resonance energy transfer.Journal of biomedical optics · 2014Article
- Spatial light modulator based active wide-field illumination for ex vivo and in vivo quantitative NIR FRET imaging.Biomedical optics express · 2014Article
- FLIM-FRET for Cancer Applications.Current molecular imagingArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Wide-field fluorescence lifetime imaging allows for fast imaging of large sample areas at the cost of low sensitivity to weak fluorescence signals. To overcome this challenge, we developed an active wide-field illumination (AWFI) strategy to optimize the impinging spatial intensity for acquiring optimal fluorescence signals over the whole sample. We demonstrated the ability of AWFI to accurately estimate lifetimes from a multiwell plate sample with concentrations ranging over two orders of magnitude. We further reported its successful application to a quantitative Förster resonance energy transfer lifetime cell-based assay. Overall, this method allows for enhanced accuracy in lifetime-based imaging at high acquisition speed over samples with large fluorescence intensity distributions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.