ArticleBMC infectious diseases2013
Comparison of the new Mycofast Revolution assay with a molecular assay for the detection of genital mycoplasmas from clinical specimens.
Article in BMC infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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13 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Genital mycoplasma infections: a hidden factor in cervical cancer progression? A systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- Diagnostic performance of rapid screening tests for asymptomatic bacteriuria among pregnant women in a tertiary care hospital in India.Scientific reports · 2026Article
- Comparative analysis of diagnostic kits for detectingPractical laboratory medicine · 2026Article
- Optimization and validation of two multiplex qPCR assays for the rapid detection of microorganisms commonly invading the amniotic cavity.Journal of reproductive immunology · 2022Article
- Evaluation of the MYCOPLASMA IST3 urogenital mycoplasma assay in an international multicentre trial.The Journal of antimicrobial chemotherapy · 2021Article
- Diagnostic parameters of the AF Genital System® for detection ofThe Journal of international medical research · 2021Observational
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- Vaginal Microbiota Profiles of Native Korean Women and Associations with High-Risk Pregnancy.Journal of microbiology and biotechnology · 2020Article
- How the Cervical Microbiota Contributes to Cervical Cancer Risk in Sub-Saharan Africa.Frontiers in cellular and infection microbiology · 2020Review
- Evidence that intra-amniotic infections are often the result of an ascending invasion - a molecular microbiological study.Journal of perinatal medicine · 2019Article
- Role of Ureaplasma Respiratory Tract Colonization in Bronchopulmonary Dysplasia Pathogenesis: Current Concepts and Update.Clinics in perinatology · 2015Review
- Antimicrobial susceptibility patterns of Ureaplasma species and Mycoplasma hominis in pregnant women.BMC infectious diseases · 2014Article
- Chlamydia trachomatis and Genital Mycoplasmas: Pathogens with an Impact on Human Reproductive Health.Journal of pathogens · 2014Review
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenital mycoplasmas are opportunistic bacteria that are associated with undesirable gynaecologic and reproductive events. Mycoplasmas are fastidious bacteria with increasing resistance to routine antimicrobials and often fail to grow on conventional culture methods. The commercial Mycofast Revolution assay permits the phenotypic detection and identification of genital mycoplasmas. Antimicrobial susceptibility testing against five antimicrobial agents with MICs corresponding to the CLSI guidelines can also be performed. This study aimed to compare the new commercially available Mycofast Revolution assay with a multiplex PCR assay.
methodsSelf-collected swabs were obtained from pregnant women attending the antenatal clinic of a tertiary academic hospital in Pretoria, South Africa from October 2012 to November 2012. These swabs were used to seed UMMt and modified Amies transport media. The seeded UMMt transported medium was used to inoculate the Mycofast Revolution assay for the identification, enumeration and antimicrobial susceptibility testing of genital mycoplasmas. Following DNA extraction from the modified Amies transport medium, specimens were subjected to a multiplex PCR assay for the detection of genital mycoplasmas.
resultsThe Mycofast Revolution kit had a sensitivity and specificity of 77.3% (95% CI: 62.15% to 88.51%) and 80% (95% CI: 28.81% to 96.70%), respectively, against the PCR assay. The positive and negative predictive values were 97.1% (95% CI: 85.03% to 99.52%) and 28.6% (95% CI: 8.57% to 58.08%). Genital mycoplasmas were detected in 71.4% (35/49) of samples with the Mycofast Revolution assay with 49% (24/49) being Ureaplasma spp. and 22.4% (11/49) mixed strains. The multiplex PCR assay had a positivity rate of 89.8% (44/49) for genital mycoplasmas; mixed strains were present in 51% (25/49) of samples, Ureaplasma spp. in 16.3% (8/49) and M. hominis in 22.4% (11/49) of samples.
conclusionsThere was a fair agreement (κ = 0.319) between the Mycofast Revolution assay and the mPCR assay. With the high prevalence rates of genital mycoplasmas, fast and efficient diagnostic methods are imperative to treat infections and minimise complications. The Mycofast Revolution assay is simple to use, has a short turn-around time and interpretation of results are straightforward. This assay circumvents common problems experienced with conventional culture and molecular methods in diagnostic laboratories where skilled personnel are limited and can be used as an alternative diagnostic assay.
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