ArticleJournal of bacteriology2013
The sll1951 gene encodes the surface layer protein of Synechocystis sp. strain PCC 6803.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 56 citations in OpenAlex.
- Systematic Evaluation of Signal Peptide-Driven Protein Secretion in the Fast-Growing CyanobacteriumBiomolecules · 2026Article
- Spatial proteomics reveals signal sequence characteristics correlated with localization in cyanobacteria.Plant physiology · 2025Article
- Rhamnose biosynthesis is not impaired by the deletion of putativeApplied and environmental microbiology · 2025Article
- Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.Folia microbiologica · 2025Article
- Interactions between the nitrogen-fixing cyanobacteriumISME communications · 2024Article
- The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803 substrains.FEBS open bio · 2023Article
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Differential near-infrared imaging of heterocysts using single-walled carbon nanotubes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Enhanced productivity of extracellular free fatty acids by gene disruptions of acyl-ACP synthetase and S-layer protein in Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2022Article
- Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803.Bioresource technology · 2022Article
- Overexpression ofInternational journal of molecular sciences · 2021Article
- Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002.Photosynthesis research · 2021Article
- Cell wall surface layer (S-layer) promotes colony formation in Microcystis: comparison of S-layer characteristics between colonial and unicellular forms of Microcystis and function conformation.Environmental science and pollution research international · 2020Article
- Extracellular Vesicles: An Overlooked Secretion System in Cyanobacteria.Life (Basel, Switzerland) · 2020Review
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- A rapid and low-cost method for genomic DNA extraction from the cyanobacteriumBiology methods & protocols · 2020Article
- Article
- Axenic Biofilm Formation and Aggregation byApplied and environmental microbiology · 2019Article
- Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.BMC biotechnology · 2019Article
- Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1.Journal of bacteriology · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sll1951 is the surface layer (S-layer) protein of the cyanobacterium Synechocystis sp. strain PCC 6803. This large, hemolysin-like protein was found in the supernatant of a strain that was deficient in S-layer attachment. An sll1951 deletion mutation was introduced into Synechocystis and was easily segregated to homozygosity under laboratory conditions. By thin-section and negative-stain transmission electron microscopy, a ~30-nm-wide S-layer lattice covering the cell surface was readily visible in wild-type cells but was absent in the Δsll1951 strain. Instead, the Δsll1951 strain displayed a smooth lipopolysaccharide surface as its most peripheral layer. In the presence of chaotropic agents, the wild type released a large (>150-kDa) protein into the medium that was identified as Sll1951 by mass spectrometry of trypsin fragments; this protein was missing in the Δsll1951 strain. In addition, Sll1951 was prominent in crude extracts of the wild type, indicating that it is an abundant protein. The carotenoid composition of the cell wall fraction of the Δsll1951 strain was similar to that of the wild type, suggesting that the S-layer does not contribute to carotenoid binding. Although the photoautotrophic growth rate of the Δsll1951 strain was similar to that of the wild-type strain, the viability of the Δsll1951 strain was reduced upon exposure to lysozyme treatment and hypo-osmotic stress, indicating a contribution of the S-layer to the integrity of the Synechocystis cell wall. This work identifies the S-layer protein in Synechocystis and shows that, at least under laboratory conditions, this very abundant, large protein has a supportive but not a critical role in the function of the cyanobacterium.
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