ArticleThe Journal of membrane biology2013
Relative CO₂/NH₃ permeabilities of human RhAG, RhBG and RhCG.
Article in The Journal of membrane biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Mutations in ammonia transporter RhBG that impair NHThe Journal of physiology · 2026Article
- Prognostic and immunological roles of ammonia-induced cell death-related genes in non-small cell lung cancer.BMC pulmonary medicine · 2026Article
- Biomimetic Organic Nanozyme as Tumor Vaccines for Targeted Suppression of Ammonia-Induced T Lymphocyte Death to Augment Breast Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Rhbg interaction with CA-IV and its effects on NHAmerican journal of physiology. Cell physiology · 2025Article
- Ammonia transport mediated by urea transporter A isoforms.Biology open · 2025Article
- Role of channels in thebioRxiv : the preprint server for biology · 2025Article
- Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8Nature cell biology · 2024Article
- Chloride Gradient Is Involved in Ammonium Influx in Human Erythrocytes.International journal of molecular sciences · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- Expression and Functional Analysis ofAnimals : an open access journal from MDPI · 2023Article
- Potential Novel Role of Membrane-Associated Carbonic Anhydrases in the Kidney.International journal of molecular sciences · 2023Review
- Architecture of the human erythrocyte ankyrin-1 complex.Nature structural & molecular biology · 2022Article
- A Rhesus channel in the coral symbiosome membrane suggests a novel mechanism to regulate NHScience advances · 2022Article
- Effects of butyratePflugers Archiv : European journal of physiology · 2022Article
- Beyond CaPflugers Archiv : European journal of physiology · 2021Article
- Carbon dioxide transport across membranes.Interface focus · 2021Review
- The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport.Pflugers Archiv : European journal of physiology · 2020Article
- Role of Carbonic Anhydrases and Inhibitors in Acid-Base Physiology: Insights from Mathematical Modeling.International journal of molecular sciences · 2019Review
- Effects of chronic hypercapnia on ammonium transport in the mouse kidney.Physiological reports · 2019Article
- Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Mammalian glycosylated rhesus (Rh) proteins include the erythroid RhAG and the nonerythroid RhBG and RhCG. RhBG and RhCG are expressed in multiple tissues, including hepatocytes and the collecting duct (CD) of the kidney. Here, we expressed human RhAG, RhBG and RhCG in Xenopus oocytes (vs. H2O-injected control oocytes) and used microelectrodes to monitor the maximum transient change in surface pH (DpHS) caused by exposing the same oocyte to 5 % CO₂/33 mM HCO₃⁻ (an increase) or 0.5 mM NH₃/NH₄⁺ (a decrease). Subtracting the respective values for day-matched, H₂O-injected control oocytes yielded channel-specific values (*). (ΔpH*(S))(CO₂) and (-ΔpH*(S))(NH₃) were each significantly >0 for all channels, indicating that RhBG and RhCG--like RhAG--can carry CO₂ and NH₃. We also investigated the role of a conserved aspartate residue, which was reported to inhibit NH₃ transport. However, surface biotinylation experiments indicate the mutants RhBG(D178N) and RhCG(D177N) have at most a very low abundance in the oocyte plasma membrane. We demonstrate for the first time that RhBG and RhCG--like RhAG--have significant CO₂ permeability, and we confirm that RhAG, RhBG and RhCG all have significant NH₃ permeability. However, as evidenced by (ΔpH*(S))(CO₂)/ (-ΔpH*(S))(NH₃) values, we could not distinguish among the CO₂/ NH₃ permeability ratios for RhAG, RhBG and RhCG. Finally, we propose a mechanism whereby RhBG and RhCG contribute to acid secretion in the CD by enhancing the transport of not only NH₃ but also CO₂ across the membranes of CD cells.
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Registered trials
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