ArticleInternational orthopaedics2014
Human osteoblast damage after antiseptic treatment.
Article in International orthopaedics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Effects of hypochlorous acid, povidone-iodine, and Carrel-Dakin solution irrigation on open fracture healing: a rat femur model.Journal of orthopaedic surgery and research · 2026Article
- Insights into the Cytotoxicity and Irritant Potential of Chlorhexidine Digluconate: An In Vitro and In Ovo Safety Screening.Dentistry journal · 2024Article
- Hydrogen Peroxide, Povidone-Iodine and Chlorhexidine Fail to EradicateLife (Basel, Switzerland) · 2023Article
- Is Chlorhexidine in Dentistry an Ally or a Foe? A Narrative Review.Healthcare (Basel, Switzerland) · 2022Review
- A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility.Journal of materials science. Materials in medicine · 2021Article
- The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study.International journal of molecular sciences · 2021Article
- Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants.Molecules (Basel, Switzerland) · 2021Article
- The Effect of Different Irrigation Solutions on the Cytotoxicity and Recovery Potential of Human Osteoblast Cells In Vitro.Arthroplasty today · 2021Article
- The effect of different irrigation solutions on fracture healing in a rat femur fracture model.Joint diseases and related surgery · 2021Article
- Sodium hypochlorite is more effective than chlorhexidine for eradication of bacterial biofilm of staphylococci and Pseudomonas aeruginosa.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2020Article
- Influence of the Surface Roughness of PEEK GRF30 and Ti6Al4V SLM on the Viability of Primary Human Osteoblasts Determined by the MTT Test.Materials (Basel, Switzerland) · 2019Article
- The downside of antimicrobial agents for wound healing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- A Comparison of the Effects of Topical Prolavacid Solution (a Polyhexamethylene Biguanide-Based Wound Cleanser) and Medihoney Ointment in a Rat Model of Cutaneous Wound.Advances in wound care · 2017Article
- Mesenchymal stromal cell and osteoblast responses to oxidized titanium surfaces pre-treated with λ = 808 nm GaAlAs diode laser or chlorhexidine: in vitro study.Lasers in medical science · 2017Article
- Irrigation Solutions Negatively Affect the Viability and Function of Human Fibroblasts: An in vitro Study.Biomedicine hubArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeAntiseptics are powerful medical agents used for wound treatment and decontamination and have a high potential for defeating joint infections in septic surgery. Both chlorhexidine and polyhexanide are frequently used in clinical practice and have a broad antimicrobial range, but their effect on human osteoblasts has not been sufficiently studied. Our objective was to investigate the toxic effects of polyhexanide and chlorhexidine on human osteoblasts in vitro to evaluate their clinical applicability in septic surgery.
methodsWe isolated and cultivated human osteoblasts in vitro and assayed the toxic effects of chlorhexidine 0.1% and polyhexanide 0.04%, concentrations commonly applied in clinical practice. Toxicity analysis was performed by visualisation of cell structure, lactate dehydrogenase (LDH) activity and evaluation of vital cells. Toxicity was evaluated by microscopic inspection of cell morphology, trypan blue staining and determination of LDH release.
resultsDamaged cell structure could be shown by microscopy. Both antiseptics promoted LDH activity after incubation with osteoblasts. The evaluation of vital osteoblasts showed a significant decrease of vital cells.
conclusionsBoth antiseptics induced significant cell death of osteoblasts at optimum exposure. We therefore recommend cautious use of polyhexanide and chlorhexidine in septic surgery to avoid severe osteoblast toxicity.
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Registered trials
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